Keratin biomaterials for cell culture and methods of use
AssigneeWake Forest University Health Sciences
InventorMark E. Van Dyke
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Abstract
Provided are keratin compositions useful in cell culture. In some embodiments the keratins are biocompatible, promote cell growth, promote cell adhesion and provide an excellent substrate for cell culture. Keratin compositions described herein may be used as coatings, gels, three-dimensional scaffolds, additives to cell culture media, microcarriers, etc. The keratin substrates may also be used to deliver cells, e.g., for cell therapy applications.
Claims20
A cell culture substrate comprising a keratin coating, said keratin coating in the form of a gel or a freeze dried gel, wherein said keratin is obtained by isolating a fraction of keratin selected from the group consisting of:
- acidic keratose, basic keratose, acidic kerateine and basic kerateine.
A method for growing cells in vitro comprising the steps of:
- contacting said cells to a cell culture substrate comprising a keratin coating, said keratin coating in the form of a gel or a freeze dried gel, wherein said keratin is obtained by isolating a fraction of keratin selected from the group consisting of:
- acidic keratose, basic keratose, acidic kerateine and basic kerateine, wherein said cells adhere to said keratin coating;
- and
- growing said cells in vitro under conditions conducive to the proliferation of said cells.
Dependencies are inferred from the claim text.