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US 9,068,018 B2Granted
Methods of using voltage-gated Hv1 proton channels to detect changes in intracellular pH
AssigneeChildren's Medical Center Corporation
InventorsIan S. Ramsey, Magdalene M. Moran, Jayhong A. Chong, David Clapham
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Abstract
Nucleic acid and protein sequences relating to a proton channel (Hv1) are disclosed. Nucleic acids, vectors, transformed cells, transgenic animals, polypeptides, and antibodies relating to the Hv1 gene and protein are disclosed. Also provided are methods of identifying modulators of Hv1 activity, methods of geno typing subjects with respect to Hv1, and methods of diagnosing and treating Hv1-mediated disorders.
Claims16
A method of detecting a change in the intracellular pH in a cell, the method comprising:
- (a) obtaining a cell transformed with an exogenous genetic construct comprising a sequence encoding a voltage-gated Hv1 proton channel proton, whereby the cell expresses the Hv1 protein having voltage-gated Hv1 proton channel proton activity;
- (b) changing the intracellular pH in the cell;
- (c) detecting a change in an electrical signal caused by the voltage-gated Hv1 proton channel protein in the cell;
- and
- (d) determining that there has been a decrease in intracellular pH in the cell based on the detection in (c) of an increase in the electrical signal caused by the voltage-gated Hv1 protein channel, or determining that there has been an increase in intracellular pH in the cell based on the detection in (c) of a decrease in the electrical signal caused by the voltage-gated Hv1 protein channel.
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