Methods and compositions for chlamydial antigens for diagnosis and treatment of chlamydial infection and disease
AssigneeBoard of Regents, The University of Texas System
InventorGuangming Zhong
Life of this patent
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Abstract
Disclosed are isolated Chlamydia trachomatis proteins, methods of fusion protein and associated antibody production, and methods of using isolated proteins and antibodies in diagnosis and detection. Also disclosed are compositions comprising isolated proteins, wherein the compositions can further comprising pharmaceutically acceptable carriers, an adjuvant and/or an immunostimulant, and methods using the pharmaceutical compositions for treating or preventing an infection by Chlamydia in a subject. The compositions may also comprise a protein or immunogenic fragment of a pathogenic organism other than Chlamydia trachomatis.
Claims18
A method of detecting an antibody to a Chlamydia protein in a sample, comprising:
- a) contacting the sample with one or more than one isolated Chlamydia trachomatis protein, said one or more than one isolated Chlamydia trachomatis protein being detectably labeled and/or immobilized on a solid support, selected from the group consisting of (1) CT828, (2) CT099, (3) CT322, (4) CT457, (5) CT005, (6) CT019, (7) CT022, (8) CT046, (9) CT067, (10) CT111, (11) CT116, (12) CT118, (15) CT208, (16) CT225, (17) CT228, (18) CT236, (19) CT237, (21) CT260, (22) CT278, (23) CT328, (24) CT376, (27) CT431, (28) CT450, (29) CT494, (30) CT507, (31) CT532, (32) CT542, (33) CT556, (34) CT578, (35) CT673, (36) CT733, (37) CT743, (38) CT759, (39) CT791, (40) CT798 and (41) CT868, an immunogenic fragment of any of (1)-(12), (15)-(19), (21)-(24) or (27)-(41), and any combination thereof, under conditions whereby an antigen/antibody complex can form;
- and
- b) detecting antigen/antibody complex formation, thereby detecting an antibody to the Chlamydia protein in the sample.
A method of diagnosing a Chlamydia infection in a subject, comprising:
- a) contacting a sample from the subject with one or more than one isolated Chlamydia trachomatis protein, said one or more than one isolated Chlamydia trachomatis protein being detectably labeled and/or immobilized on a solid support, selected from the group consisting of (1) CT828, (2) CT099, (3) CT322, (4) CT457, (5) CT005, (6) CT019, (7) CT022, (8) CT046, (9) CT067, (10) CT111, (11) CT116, (12) CT118, (15) CT208, (16) CT225, (17) CT228, (18) CT236, (19) CT237, (21) CT260, (22) CT278, (23) CT328, (24) CT376, (27) CT431, (28) CT450, (29) CT494, (30) CT507, (31) CT532, (32) CT542, (33) CT556, (34) CT578, (35) CT673, (36) CT733, (37) CT743, (38) CT759, (39) CT791, (40) CT798 and (41) CT868, an immunogenic fragment of any of (1)-(12), (15)-(19), (21)-(24) or (27)-(41), and any combination thereof under conditions whereby an antigen/antibody complex can form;
- and
- b) detecting antigen/antibody complex formation, thereby detecting an antibody to a Chlamydia protein in the sample, wherein the Chlamydia protein is selected from the group consisting of (1) CT828, (2) CT099, (3) CT322, (4) CT457, (5) CT005, (6) CT019, (7) CT022, (8) CT046, (9) CT067, (10) CT111, (11) CT116, (12) CT118, (15) CT208, (16) CT225, (17) CT228, (18) CT236, (19) CT237, (21) CT260, (22) CT278, (23) CT328, (24) CT376, (27) CT431, (28) CT450, (29) CT494, (30) CT507, (31) CT532, (32) CT542, (33) CT556, (34) CT578, (35) CT673, (36) CT733, (37) CT743, (38) CT759, (39) CT791, (40) CT798 and (41) CT868, and thereby diagnosing a Chlamydia infection in the subject.
A method of detecting a Chlamydia protein in a sample, comprising:
- a) contacting the sample with an antibody that specifically binds a Chlamydia protein or immunogenic fragment thereof, said antibody that specifically binds a Chlamydia protein or immunogenic fragment thereof being detectably labeled and/or immobilized on a solid support, wherein the Chlamydia protein is selected from the group consisting of (1) CT828, (2) CT099, (3) CT322, (4) CT457, (5) CY005, (6) CT019, (7) CT022, (8) CT046, (9) CT067, (10) CT111, (11) CT116, (12) CT118, (15) CT208, (16) CT225, (17) CT228, (18) CT236, (19) CT237, (21) CT260, (22) CT278, (23) CT328, (24) CT376, (27) CT431, (28) CT450, (29) CT494, (30) CT507, (31) CT532, (32) CT542, (33) CT556, (34) CT578, (35) CT673, (36) CT733, (37) CT743, (38) CT759, (39) CT791, (40) CT798 and (41) CT868, under conditions whereby an antigen/antibody complex can form;
- and
- b) detecting antigen/antibody complex formation, thereby detecting the Chlamydia protein in the sample.
A method of diagnosing a Chlamydia infection in a subject, comprising:
- a) contacting a sample from the subject with an antibody that specifically binds a Chlamydia protein or immunogenic fragment thereof, said antibody that specifically binds a Chlamydia protein or immunogenic fragment thereof being detectably labeled and/or immobilized on a solid support, wherein the Chlamydia protein is selected from the group consisting of (1) CT828, (2) CT099, (3) CT322, (4) CT457, (5) CT005, (6) CT019, (7) CT022, (8) CT046, (9) CT067, (10) CT111, (11) CT116, (12) CT118, (15) CT208, (16) CT225, (17) CT228, (18) CT236, (19) CT237, (21) CT260, (22) CT278, (23) CT328, (24) CT376, (27) CT431, (28) CT450, (29) CT494, (30) CT507, (31) CT532, (32) CT542, (33) CT556, (34) CT578, (35) CT673, (36) CT733, (37) CT743, (38) CT759, (39) CT791, (40) CT798 and (41) CT868, under conditions whereby an antigen/antibody complex can form;
- and
- b) detecting antigen/antibody complex formation, thereby detecting a Chlamydia protein, wherein the Chlamydia protein is selected from the group consisting of (1) CT828, (2) CT099, (3) CT322, (4) CT457, (5) CT005, (6) CT019, (7) CT022, (8) CT046, (9) CT067, (10) CT111, (11) CT116, (12) CTII8, (15) CT208, (16) CT225, (17) CT228, (18) CT236, (19) CT237, (21) CT260, (22) CT278, (23) CT328, (24) CT376, (27) CT431, (28) CT450, (29) CT494, (30) CT507, (31) CT532, (32) CT542, (33) CT556, (34) CT578, (35) CT673, (36) CT733, (37) CT743, (38) CT759, (39) CT791, (40) CT798 and (41) CT868, and thereby diagnosing a Chlamydia infection in the subject.
A method of eliciting an immune response to a Chlamydia protein in a subject, comprising:
- administering to the subject an effective amount of a Chlamydia protein or an immunogenic fragment thereof, wherein the Chlamydia protein is selected from the group consisting of (1) CT828, (2) CT099, (3) CT322, (4) CT457, (5) CT005, (6) CT019, (7) CT022, (8) CT046, (9) CT067, (10) CT111, (11) CT116, (12) CT118, (15) CT208, (16) CT225, (17) CT228, (18) CT236, (19) CT237, (21) CT260, (22) CT278, (23) CT328, (24) CT376, (27) CT431, (28) CT450, (29) CT494, (30) CT507, (31) CT532, (32) CT542, (33) CT556, (34) CT578, (35) CT673, (36) CT733, (37) CT743, (38) CT759, (39) CT791, (40) CT798, (41) CT868, and any combination thereof, thereby eliciting an immune response to the Chlamydia protein in the subject.
A method of treating an infection by Chlamydia in a subject or preventing a disease or disorder caused by an infection by Chlamydia in a subject, comprising administering to the subject an effective amount of an isolated Chlamydia protein or immunogenic fragment thereof, wherein the Chlamydia protein is selected from the group consisting of (1) CT828, (2) CT099, (3) CT322, (4) CT457, (5) CT005, (6) CT019, (7) CT022, (8) CT046, (9) CT067, (10) CT111, (11) CT116, (12) CT118, (15) CT208, (16) CT225, (17) CT228, (18) CT236, (19) CT237, (21) CT260, (22) CT278, (23) CT328, (24) CT376, (27) CT431, (28) CT450, (29) CT494, (30) CT507, (31) CT532, (32) CT542, (33) CT556, (34) CT578, (35) CT673, (36) CT733, (37) CT743, (38) CT759, (39) CT791, (40) CT798 and (41) CT868, thereby treating an infection by Chlamydia in a subject or preventing a disease or disorder caused by an infection by Chlamydia in the subject.
A method of reducing the likelihood of infertility due to Chlamydia infection in a subject, comprising administering to the subject an effective amount of an isolated Chlamydia protein or immunogenic fragment thereof, wherein the Chlamydia protein is selected from the group consisting of (1) CT828, (2) CT099, (3) CT322, (4) CT457, (5) CT005, (6) CT019, (7) CT022, (8) CT046, (9) CT067, (10) CT111, (11) CT116, (12) CT118, (15) CT208, (16) CT225, (17) CT228, (18) CT236, (19) CT237, (21) CT260, (22) CT278, (23) CT328, (24) CT376, (27) CT431, (28) CT450, (29) CT494, (30) CT507, (31) CT532, (32) CT542, (33) CT556, (34) CT578, (35) CT673, (36) CT733, (37) CT743, (38) CT759, (39) CT791, (40) CT798 and (41) CT868, thereby reducing the likelihood of infertility due to Chlamydia infection in the subject.
A method of reducing the incidence of hydrosalpinx due to Chlamydia infection in a subject, comprising administering to the subject a composition comprising an effective amount of an isolated Chlamydia protein or immunogenic fragment thereof, wherein the Chlamydia protein is selected from the group consisting of (1) CT828, (2) CT099, (3) CT322, (4) CT457, (5) CT005, (6) CT019, (7) CT022, (8) CT046, (9) CT067, (10) CT111, (11) CT116, (12) CT118, (15) CT208, (16) CT225, (17) CT228, (18) CT236, (19) CT237, (21) CT260, (22) CT278, (23) CT328, (24) CT376, (27) CT431, (28) CT450, (29) CT494, (30) CT507, (31) CT532, (32) CT542, (33) CT556, (34) CT578, (35) CT673, (36) CT733, (37) CT743, (38) CT759, (39) CT791, (40) CT798 and (41) CT868, thereby reducing the incidence of hydrosalpinx due to Chlamydia infection in the subject.
Dependencies are inferred from the claim text.