Glycated CD59 peptides, their preparation, and uses thereof
AssigneePresident and Fellows of Harvard College
InventorsJose A. Halperin, Michael Chorev
Life of this patent
Estimating the term…
Abstract
The present invention provides glycated Amadori products of the CD59 peptide and fragments thereof to be used as tools and among methods for the diagnosis and prognosis of pre-diabetes and diabetes. Certain aspects of the invention include glycated Amadori products of CD59 and fragments thereof to be used for the generation of antibodies and antibody fragments. Still other aspects of the invention include methodologies for the preparation of glycated Amadori products of CD59, fragments thereof, the inventive antibodies, and antibody fragments.
Claims3
A peptide comprising a segment of CD59 of the formula:
- or a protected form thereof, wherein each sidechain of each amino acid residue of said peptide optionally comprises an
- group;
- wherein
- each R1 is independently hydrogen or Pg1 and wherein two Pg1 groups may combine to form a heterocyclic ring;
- each X is O or NH;
- each R6 is independently hydrogen or Pg2;
- each R7 is independently hydrogen;
- Pg2, or Pg3;
- each R8 is independently hydrogen or Pg4;
- each Pg1 is an independently selected hydroxyl protecting group;
- each Pg2 is an independently selected amino protecting group;
- each Pg3 is an independently selected carboxyl protecting group;
- and
- each Pg4 is an independently selected thiol protecting group.
A substantially pure peptide comprising a segment of CD59 of the formula:
- or a protected form thereof, wherein each sidechain of each amino acid residue of said peptide optionally comprises an
- group;
- wherein
- each R1 is independently hydrogen or Pg1 and wherein two Pg1 groups may combine to form a heterocyclic ring;
- each X is O or NH;
- each R6 is independently hydrogen or Pg2;
- each R7 is independently hydrogen;
- Pg2, or Pg3;
- each R8 is independently hydrogen or Pg4;
- each Pg1 is an independently selected hydroxyl protecting group;
- each Pg2 is an independently selected amino protecting group;
- each Pg3 is an independently selected carboxyl protecting group;
- and
- each Pg4 is an independently selected thiol protecting group.
Dependencies are inferred from the claim text.