Protein purification using displacement chromatography
AssigneeAbbVie Inc.
InventorsChen Wang, Germano Coppola, Chris Chumsae
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Abstract
Disclosed herein are compositions and methods for the isolation and purification of proteins from a sample. In particular, the present invention relates to compositions and methods for isolating and purifying proteins incorporating a displacement chromatographic step. The present invention is also directed toward pharmaceutical compositions comprising one or more antibodies purified by a method described herein.
Claims30
A method for producing an antibody composition comprising a reduced amount of acidic species of the antibody, wherein the antibody comprises the heavy and light chains of adalimumab, the method comprising:
- (a) contacting a first composition comprising the antibody with a chromatography media, wherein the first composition comprises more than 10% total acidic species of adalimumab,
- wherein the acidic species of the antibody comprise species selected from the group consisting of charge variants, size variants, fragmentation variants, and any combinations thereof,
- wherein the acidic species of the antibody do not include process-related impurities selected from the group consisting of host cell proteins, host cell DNA, and media components,
- wherein the antibody binds to the chromatography media;
- (b) displacing the antibody bound to the chromatography media with a displacing buffer;
- and
- (c) collecting a second composition comprising the displaced antibody, wherein the second composition comprises less than 10% total acidic species of the antibody,
- thereby producing an antibody composition comprising a reduced amount of acidic species of the antibody.
Dependencies are inferred from the claim text.