Compositions and methods for redox modulated proteins
OwnerSrikanth Vedamoorthyinventor
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Abstract
Disclosed embodiments relate to antibodies that recognize redox modulated proteins such as glutathionylated actin. Embodiments relate to a reagent for the immunoassay of glutathionylated actin. Disclosed embodiments also relate to diagnosis of and therapies for the treatment of diseases related to impaired deglutathionylation of peptides. Additionally, disclosed embodiments relate to pharmaceutical compositions containing antibodies which recognize redox modulated proteins and kits for the detection or treatment of diseases related to redox modulated proteins which include antibodies that recognize redox modulated proteins.
Claims6
A method for synthesizing an immunogen, comprising the steps of:
- selecting a peptide useful for immunization;
- adding the selected peptide to a buffer solution containing stoichiometric excesses of glutathione and aldrithiol, under constant stirring at room temperature, resulting in a glutathionylated peptide;
- wherein the selected peptide is actin;
- selecting a vaccine carrier protein;
- and conjugating the glutathionylated peptide to a vaccine carrier protein.
Dependencies are inferred from the claim text.