Chemiluminescent compositions, methods, assays and kits for oxidative enzymes

A dioxetane of the form (I):

• where T is
• wherein R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron withdrawing groups or electron-donating groups, or groups providing oxidative isozyme substrate recognition, and
• wherein R1 comprises an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms;
• and
• wherein R2, R3, R4, and R6 can be independently H, or are selected from the group consisting of halo (F, Cl, Br, I), trialkylammonium (—NR3+), alkylamido (—NHCOR, (—NRCOR), arylamido (—NHCOAr, NRCOAr, NArCOAr), arylcarbamoyl (—NHCOOAr, —NRCOOAr), alkylcarbamoyl (—NHCOOR, NRCOOR), cyano (—CN), nitro (—NO2), ester (—COOR, COOAr), alkyl- or arylsulfonamido (—NHSO2R, —NHSO2Ar), trifluoromethyl (—CF3), alkyl (—R), aryl (—Ar), alkyl- or arylamidosulfonyl (—SO2NHCOR, —SO2NHCOAr), alkyl- or arylsulfonyl (—SO2R, —SO2Ar), alkyl- or arylthioethers (—SR, —SAr), alkoxy (—OR), or aryloxy (—OAr) substituents, and
• wherein R5 is a group that can be removed upon activation by an oxidative enzyme;
• and
• wherein X is selected from the group consisting of S, N and O.