US 9,066,960 B2Granted

Use of the effective fraction of alkaloids from mulberry twig in preparing hypoglycemic agents

AssigneeInstitute of Mataria Medica, Chinese Academy of Medical Sciences

InventorsYuling Liu, Zhufang Shen, Zhen Chen, Renyun Wang, Xuejun Xia, Yueteng Chen, Quan Liu, Sujuan Sun, Mingzhi Xie

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Abstract

The present invention relates to an effective fraction of alkaloids and the effective fraction is prepared from mulberry twig and its active ingredients are a composition of alkaloids. Determined by HPLC, the percentage of the total alkaloids are 50% or more by weight in the effective fraction and the percentage of the compound 1-deoxynojirimycin is 30% or more by weight in the total alkaloids. The effective fraction of the invention is prepared as the following steps: the mulberry twig is extracted by solvents, and the extract is precipitated by alcohol precipitation or flocculation to remove the impurities, and then concentrated, purified by resin chromatography. The present invention also relates to a pharmaceutical composition containing the said effective fraction of alkaloids and to the use of the effective fraction of alkaloids according to claim 1-4 in preparing hypoglycemic agents.

Claims15

Dependent claims

A fraction of alkaloids having hypoglycemic activity, prepared by a process comprising:

(a) extracting mulberry twig with solvents and precipitating the extract solution;
(b) collecting the supernatant fluid, adding the collected supernatant fluid into cation exchange resin, and eluting the resin with a weak base eluent;
and
(c) collecting and concentrating the effluent, adding the concentrated effluent into anion exchange resin, collecting the part not absorbed to the anion exchange resin, and drying the collected part to obtain the fraction of alkaloids having hypoglycemic activity in powder,
wherein the fraction of alkaloids having hypoglycemic activity comprises as the active ingredients a mixture of alkaloids, and wherein the percentage of the total alkaloids are 50% or more by weight in the fraction of alkaloids having hypoglycemic activity and the percentage of the compound 1-deoxynojirimycin is 30% or more by weight in the total alkaloids, and wherein hypoglycemic activity of the fraction as determined by the IC50 of sucrase is 21.9 ng/ml.

2 more elements

Dependencies are inferred from the claim text.