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US 9,066,909 B2Granted
Methods for producing and using rejuvenated red blood cells
AssigneeBiomet Biologics, LLC
InventorAlan Gray
Life of this patent
Estimating the term…
Abstract
Methods for treating blood. The methods comprise obtaining whole blood from a donor, mixing the whole blood with a red blood cell enhancement composition, and incubating the mixture of whole blood and red blood cell enhancement composition. The method can be performed at a time proximate to the time of obtaining blood from the donor, or after the blood has been stored for a period of time. The method rejuvenates red blood cells.
Claims11
A method for restoring a metabolic profile in red blood cells (RBCs), comprising:
- a) obtaining whole blood from a donor;
- b) removing leukocytes from the whole blood, to produce a volume of leukoreduced blood;
- c) mixing the leukoreduced blood with an RBC enhancement composition comprising a rejuvenating material selected from the group consisting of inosine, adenine, pyruvate, phosphate, and mixtures thereof;
- and
- d) incubating the mixture of leukoreduced blood and RBC enhancement composition, thereby restoring the metabolic profile in the RBCs and forming treated leukoreduced RBCs;
- and
- e) administering the treated leukoreduced RBCs to a subject in need thereof,
- wherein the levels of one or both of adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG) in the treated leukoreduced blood after the step of incubating are restored to levels equal to or greater than the levels of ATP and 2,3-DPG found in fresh blood, and wherein the removing, mixing, incubating, and administering are performed within 24 hours of the obtaining of the whole blood from the donor.
5 more elements
A method for treating a disorder characterized by reduced tissue oxygenation in a mammalian subject, comprising:
- a) obtaining whole blood from a donor;
- b) removing leukocytes from the whole blood, to produce a volume of leukoreduced blood;
- c) mixing the leukoreduced blood with an RBC enhancement composition comprising inosine, adenine, pyruvate, and phosphate;
- d) incubating the mixture of leukoreduced blood and RBC enhancement composition, thereby restoring the metabolic profile in the RBCs and forming treated leukoreduced RBCs;
- and
- e) administering the incubated blood to the subject
- wherein the levels of one or both of adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG) in the treated leukoreduced blood after the step of incubating are restored to levels equal to or greater than the levels of ATP and 2,3-DPG found in fresh blood, and wherein the removing, mixing, incubating, and administering are performed within 24 hours of the obtaining of the whole blood from the donor.
4 more elements
Dependencies are inferred from the claim text.