Interferon-β production modulating listeria strains and methods for using same
AssigneeThe Regents of the University of California
InventorsDaniel A. Portnoy, Anat A. Herskovits, Gregory Crimmins
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Abstract
Mutant Listeria bacteria that modulate interferon-B production are provided. The subject bacteria are characterized by having a mutation in a gene chosen from a TetR gene, a LadR gene, a VirR gene, a MarR gene a MdrL gene, a MdrT gene and a MdrM gene. The subject bacteria find use in a variety of applications, where representative applications of interest include, but are not limited to: (a) use of the subject bacteria as adjuvants; (b) use of the subject bacteria as delivery vectors for introducing macromolecules into a cell; (c) use of the subject bacteria as vaccines for eliciting or boosting a cellular immune response; etc.
Claims22
An attenuated Listeria bacterium comprising a mutation which modulates the expression of a multidrug resistance transporter, wherein said Listeria bacterium modulates interferon-β production in macrophages and when said mutation is a mutation in a transcription regulator gene, the mutation is a mutation in the TetR, LadR or MarR gene.
A vaccine comprising an attenuated Listeria bacterium having a mutation which modulates the expression of a multidrug resistance transporter, wherein said attenuated Listeria bacterium modulates interferon-β production in macrophages and when said mutation is a mutation in a transcription regulator gene, the mutation is a mutation in the TetR, LadR or MarR gene.
A method of eliciting or boosting a cellular immune response in a subject, said method comprising:
- administering to said subject an effective amount of a vaccine comprising an attenuated Listeria bacterium having a mutation which modulates the expression of a multidrug resistance transporter, wherein said attenuated Listeria bacterium modulates interferon-β production in macrophages.
A Listeria bacterium comprising a mutation which modulates the expression of a multidrug resistance transporter, wherein said Listeria bacterium modulates interferon-β production in macrophages and expresses a heterologous antigen that provides protection in an animal against challenge by the infectious agent from which the heterologous antigen was derived or which modulates tumor growth and metastasis in a beneficial manner.
A Listeria bacterium comprising a mutation which modulates the expression of a multidrug resistance transporter, wherein said Listeria bacterium modulates interferon-β production in macrophages and comprises a mutation in a transcription regulator gene and a multidrug resistance transporter gene.
Dependencies are inferred from the claim text.