Advanced functional biocompatible polymeric matrix containing nano-compartments

A biopolymeric film, comprising:

• a bioactive chitosan sponge-like film comprising one or more hydrophobic moieties covalently attached to the chitosan, wherein said chitosan sponge-like film is lyophilized and wherein said film includes nano-compartmentalized vesicles loaded with bioactive agent, the vesicles bound to hydrophobically modified chitosan scaffolds through the hydrating of the lyophilized film of hydrophobically modified chitosan scaffolds with a solution of the vesicles.

A kit, comprising:

• a lyophilized chitosan sponge-like film comprising hydrophobic moieties, and one or more vesicles comprising bioactive agents, wherein said vesicles are capable of attaching to said hydrophobic moieties.

A method of fabricating a biopolymeric film, comprising:

• covalently binding a chitosan biopolymer with amphiphilic, organic compounds;
• and dehydrating the hydrophobically modified biopolymer into a solid state film through a lyophilization process.

A method of using a hydrophobically modified biopolymer film, comprising:

• hydrophobically interacting a chitosan sponge-like film, comprising one or more hydrophobic moieties covalently attached to the chitosan, wherein said chitosan sponge-like film is lyophilized, with vesicles loaded with bioactive agents and allowing the self-assembly of a functionalized biopolymeric networked film with the vesicles.