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US 9,066,866 B2Granted
Direct cellular energy delivery system
AssigneeUniversity of Louisville Research Foundation, Inc.
InventorsWilliam D. Ehringer, Sufan Chien
Life of this patent
Estimating the term…
Abstract
A lipid vesicle comprising a phospholipid which is a stable vesicle former and at least one unstable vesicle forming member, wherein the unstable vesicle forming member is selected from the group consisting of a polar lipid which is not a stable vesicle former, a PEG, a raft former and a fusion protein is provided. The vesicle can further comprise a biomolecule, such as for example ATP. Methods of using the vesicle for delivery of biomolecules are also provided.
Claims51
Vesicles, comprising:
- (a) a first lipid that is a phosphatidylcholine;
- and
- (b) a second lipid having a structure selected from the group consisting of formulas
- (XXIV), (XXV), (XXVI), (XXVII), (XXIX), (XXXI), (XXXII), (XXXIII), and (XXXIV):
- and
- a high energy phosphate selected from the group consisting of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), cytosine triphosphate (CTP), cytosine diphosphate (CDP), cytosine monophosphate (CMP), uracil triphosphate (UTP), uracil diphosphate (UDP), uracil monophosphate (UMP), guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GMP), thymine triphosphate (TTP), thymine diphosphate (TDP), thymine monophosphate (TMP), inosine triphosphate (ITP), inosine diphosphate (IDP), inosine monophosphate (IMP), and phosphocreatine;
- and
- wherein the vesicles are unilamellar, and wherein the vesicles an absorption rate of at least 1 vesicle absorption per second per cell when the vesicles are contacted with target cells.
5 more elements
A vesicle, comprising:
- adenosine triphosphate (ATP);
- soy phosphatidylcholine;
- and
- 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP),
- wherein the vesicle is unilamellar.
2 more elements
A vesicle, comprising:
- adenosine triphosphate (ATP);
- 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) and
- 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP),
- wherein the vesicle is unilamellar.
1 more element
A vesicle, comprising:
- A first lipid that is a phosphatidylcholine;
- A second lipid having the structure of formula XXXII
- and
- a high energy phosphate selected from the group consisting of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), cytosine triphosphate (CTP), cytosine diphosphate (CDP), cytosine monophosphate (CMP), uracil triphosphate (UTP), uracil diphosphate (UDO), uracil monophosphate (UMP), guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GMP), thymine triphosphate (TTP), thymine diphosphate (TDP), thymine monophosphate (TMP), inosine triphosphate (ITP), inosine diphosphate (IDP), inosine monophosphate (IMP), and phosphocreatine;
- and
- wherein the vesicle is unilamellar.
3 more elements
A vesicle, comprising:
- a high energy phosphate selected from the group consisting of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), cytosine triphosphate (CTP), cytosine diphosphate (CDP), cytosine monophosphate (CMP), uracil triphosphate (UTP), uracil diphosphate (UDP), uracil monophosphate (UMP), guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GMP), thymine triphosphate (TTP), thymine diphosphate (TDP), thymine monophosphate (TMP), inosine triphosphate (ITP), inosine diphosphate (IDP), inosine monophosphate (IMP), and phosphocreatinea phosphatidylcholine;
- and
- 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP),
- wherein the vesicle is unilamellar.
1 more element
Dependencies are inferred from the claim text.