Methods and systems of optical imaging for target detection in a scattering medium
AssigneeGeneral Electric Company
InventorsSiavash Yazdanfar, Floribertus Heukensfeldt Jansen, Stephen Lomnes, Xinghua Wang
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Abstract
A method for enhancing contrast in fluorescence imaging is provided. The method comprises providing a patterned illumination source for illuminating one or more regions corresponding to a scan step, scanning at least a portion of a surface of a subject using a plurality of scan steps, acquiring image frames corresponding to two or more scan steps, deducting a background fluorescence from the image frames corresponding to the two or more scan steps to form one or more processed image frames, and reconstructing an image using one or more of the processed image frames.
Claims20
A method for enhancing contrast in fluorescence imaging, comprising:
- providing a patterned illumination source for illuminating one or more regions corresponding to a scan step;
- scanning at least a portion of a surface of a subject using a plurality of scan steps;
- collecting an emitted light from an illuminated region as well as from an area of interest away from the illuminated region;
- acquiring image frames corresponding to two or more scan steps;
- deducting a background fluorescence from the image frames corresponding to the two or more scan steps to form one or more processed image frames, wherein the background fluorescence is based on an excitation spread function;
- and
- reconstructing an image using one or more of the processed image frames.
A fluorescence imaging system for generating an enhanced image of a target in a subject, comprising:
- a source configured to illuminate at least a portion of a surface of the subject by a patterned illumination at one or more scan steps;
- an imaging detector configured to acquire image frames at one or more scan steps, wherein the imaging detector is configured to collect emitted light from an illuminated region as well as from an area of interest away from the illuminated region;
- and
- an imaging processor configured to process the image frames to deduct a background fluorescence from the image frames and reconstruct an image of the target using the processed image frames, wherein the background fluorescence is based on an excitation spread function.
Dependencies are inferred from the claim text.