Listeria bacteriophage tailspike protein and uses thereof
AssigneeBiomerieux
InventorsHolger Grallert, Sonja Molinaro, Julia Lorenz
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Abstract
The present invention relates to a novel tail spike protein (TSP) encoded by the novel Listeria bacteriophage designated ProCC P825 and uses of the novel TSP for identifying, detecting and monitoring of Listeria.
Claims18
An isolated chimeric polypeptide comprising a P825Orf2 amino acid sequence at least 75% identical to the amino acid sequence of SEQ ID NO:
- 2, wherein the polypeptide has binding specificity for Listeria cells fused to a heterologous polypeptide.
An expression vector comprising a nucleic acid sequence encoding a polypeptide comprising a P825Orf2 amino acid sequence at least 75% identical to the amino acid sequence of SEQ ID NO:
- 2, wherein the polypeptide has binding specificity for Listeria cells.
An in vitro method for detecting Listeria comprising:
- (a) contacting a sample suspected of containing Listeria with a polypeptide comprising a P825Orf2 amino acid sequence at least 75% identical to the amino acid sequence of SEQ ID NO:
- 2, wherein the polypeptide has binding specificity for Listeria cells;
- and
- (b) detecting the Listeria bound to the polypeptide.
A kit for detecting Listeria comprising an isolated polypeptide comprising a P825Orf2 amino acid sequence at least 75% identical to the amino acid sequence of SEQ ID NO:
- 2, wherein the polypeptide has binding specificity for Listeria cells, wherein the polypeptide is immobilized on a solid support.
Dependencies are inferred from the claim text.