Method and assay for detection of residues
AssigneeCharm Sciences, Inc.
InventorsSteven J. Saul, Robert J. Markovsky, David W. Douglas
Life of this patent
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Abstract
Embodiments described herein include methods and assays for detecting an analyte in a sample using a plurality of control zone capture agents. Some embodiments include detection of multiple analytes in a sample utilizing a plurality of analyte binders and a control zone containing multiple control zone capture agents. In some embodiments, the multiple control zone capture agents capture a plurality of binders within one control zone. Test results are determined by comparison of the control zone signal to a test zone signal.
Claims27
In a lateral flow assay for detecting a plurality of analytes in a solution, an assay comprising:
- (a) a test solution having a first set of binders and a second set of binders distinct from the first set of binders, each set of binders configured for generating a detectable signal, wherein binders from the first set combine with a beta-lactam from the solution, when present, to form a binder-beta-lactam complex and binders from the second set combine with an aflatoxin, when present, to form a binder-aflatoxin complex, and wherein a test solution contains a combination of one or more binders from each set, a binder-beta-lactam complex, and a binder-aflatoxin complex;
- (b) a test strip configured to allow the test solution to flow, the test strip comprising:
- (i) a first test zone having immobilized thereon a first test zone capture agent specific to the first set of binders that, when the binder has not formed a binder-beta-lactam complex, captures at least one of the binders from that set;
- (ii) a second test zone having immobilized thereon a second test zone capture agent specific to the second set of binders that, when the binder has not formed a binder-aflatoxin complex, captures at least one of the binders from that set, and wherein the second test zone being adjacent to the first test zone;
- (iii) a single control zone having a first and second set of control zone capture agents which are able to capture, at the control zone, at least one of a corresponding set of binders, whether or not the binder has formed a corresponding binder-beta-lactam or binder-aflatoxin complex, and
- wherein capture of a binder at either of the test zones or the control zone results in a detectable signal and wherein a greater signal in the control zone as compared to any one of the test zones indicates a positive result.
In a lateral flow assay for detecting a plurality of analytes in a solution, an assay comprising:
- (a) a test solution having a first set of binders and a second set of binders distinct from the first set of binders, each set of binders configured for generating a detectable signal, wherein binders from the first set combine with a beta-lactam from the solution, when present, to form a binder-beta-lactam complex and binders from the second set combine with an aflatoxin, when present, to form a binder-aflatoxin complex, and wherein a test solution contains a combination of one or more binders from each set, a binder-beta-lactam complex, and a binder-aflatoxin complex;
- (b) a test strip configured to allow the test solution to flow, the test strip comprising:
- (i) a plurality of first test zone having immobilized thereon a first test zone capture agent specific to the first set of binders that, when the binder has not formed a binder-beta-lactam complex, captures at least one of the binders from that set;
- (ii) a second test zone having immobilized thereon a second test zone capture agent specific to the second set of binders that, when the binder has not formed a binder-aflatoxin complex, captures at least one of the binders from that set, and wherein the second test zone being adjacent to the first test zone;
- (iii) a single control zone having a first and second set of control zone capture agents,
- wherein capture of a binder at either the test zones results in a detectable signal and wherein a greater signal in the control zone as compared to any one of the test zones indicates a positive result.
Dependencies are inferred from the claim text.