Method for analysis using X-ray fluorescence
AssigneeXrpro Sciences, Inc.
InventorsLori J. Peterson, Cameron A. Mortillaro, Benjamin P. Warner, Nathan H. Zahler, Pratima Bharti, Chang-Tai Hsieh, Emilia A. Solomon
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Abstract
The present invention is a method to quantify biomarkers. The method uses an X-ray fluorescence spectrometer to perform an X-ray fluorescence analysis on the sample to obtain spectral features derived from the biomarker; and quantifying the X-ray fluorescence signal of the biomarker.
Claims14
A method to quantify biomarkers, comprising providing a sample comprising a biomarker, the biomarker comprising one or more chemicals that are related to a physiological condition;
- using an X-ray fluorescence spectrometer to perform an X-ray fluorescence analysis on the sample to obtain spectral features derived from the biomarker;
- and quantifying the X-ray fluorescence signal of the biomarker;
- wherein the X-ray fluorescence spectrometer irradiates the sample with polychromatic X-rays, and wherein the sample is exposed to a solution that is substantially free of at least one element selected from the list of phosphorus and sulfur.
A method to diagnose abnormal conditions, comprising providing one or more clinical samples, the one or more clinical samples comprising a biomarker;
- using an X-ray fluorescence spectrometer to perform an X-ray fluorescence analysis on at least one of the one or more clinical samples to obtain spectral features derived from at least one biomarker;
- and quantifying the X-ray fluorescence signal of the biomarker, wherein the X-ray fluorescence spectrometer irradiates the sample with polychromatic X-rays, and wherein the one or more clinical samples are exposed to a solution that is substantially free of at least one element selected from the list of phosphorus and sulfur.
A method to identify one or more abnormal portions of a sample, comprising providing a clinical sample disposed on or in a sample holder, the clinical sample comprising normal and abnormal portions;
- providing an X-ray fluorescence spectrometer, the X-ray fluorescence spectrometer comprising a X-ray excitation source;
- using the X-ray fluorescence spectrometer to perform an X-ray fluorescence analysis on the clinical sample to obtain spectral features derived from at least one biomarker;
- and using spectral features derived from the biomarker to determine whether at least one portion of the sample is normal or abnormal, wherein
- the X-ray fluorescence is performed using excitation of the sample with polychromatic X-rays, and wherein the X-ray fluorescence analysis comprises multiple X-ray fluorescence analyses obtained wherein the sample is disposed at at least two different angles relative to the excitation source.
Dependencies are inferred from the claim text.