Determination of the absence or presence of Bacillus cereus enterotoxin via a western blot
AssigneeScientific Protein Laboratories, LLC
InventorsDustin Nielsen, Rachel Wezeman, Matthew Bristol, Mark Eifler, Lisa Petersen, Erik Walke, Lin Rao
Life of this patent
Estimating the term…
Abstract
Disclosed is a method for the determination of the absence or presence of bacillus cereus enterotoxin in a protein sample via a western blot.
Claims10
A method for determining the absence or presence of one or more components of Bacillus Diarrheal Enterotoxin (BDE) in a pancreatin Active Pharmaceutical Ingredient (API) sample comprising separating the one or more components of BDE from the pancreatin API sample by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and identifying one or more components of BDE by using at least one probe antibody specific for at least one or more components of BDE;
- wherein the one or more components of BDE are selected from the group consisting of Hemolysin BL (Hbl), Nonhemolytic Enterotoxin (Nhe), Cytotoxin K (CytK) and Enterotoxin T (BcET).
Dependencies are inferred from the claim text.