Method of producing human-like glycosylation pattern using cells deficient in glutamine synthase, CMP-N-acetylneuraminic acid hydroxylase and/or glycoprotein alpha-1,3-galactosyltransferase
AssigneeSigma-Aldrich Co.
InventorsNan Lin, Henry J. George, Joaquina Mascarenhas, Trevor N. Collingwood, Kevin J. Kayser, Katherine Achtien
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Abstract
The present invention provides non-human mammalian cell lines that are deficient in CMP-Neu5Ac hydroxylase (Cmah) and/or glycoprotein alpha-1,3-galactosyltransferase (Ggta1). Also provided are methods for using the cells disclosed herein for producing recombinant proteins with human-like patterns of glycosylation.
Claims11
A method for producing a recombinant protein with a human-like glycosylation pattern, the method comprising expressing the recombinant protein in a Chinese Hamster Ovary cell (CHO) cell line deficient in glutamine synthase, cytidine monophosphate-N-acetylneuraminic acid hydroxylase (Cmah) and glycoprotein alpha-1,3-galactosyltransferase-1 (Ggtal).
Dependencies are inferred from the claim text.