US 9,062,332 B2Granted

Process for the fermentative preparation of sulphur-containing amino acids

AssigneeEvonik Degussa GmbH

InventorsFrank Schneider, Stella Molck, Brigitte Bathe

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Abstract

Disclosed herein is a process for the fermentative preparation of sulphur-containing amino acids chosen from the group of L-methionine, L-cysteine, L-cystine, L-homocysteine and L-homocystine, comprising the steps: a) provision of a microorganism of the family Enterobacteriaceae or of a microorganism of the family Corynebacteriaceae which has an increased thiosulphate sulphurtransferase activity compared with the particular starting strain; b) fermentation of the microorganism from a) in a medium which contains an inorganic source of sulphur chosen from the group of salt of dithiosulphuric acid or a mixture of a salt of dithiosulphuric acid and a salt of sulphuric acid, a fermentation broth being obtained, and c) concentration of the sulphur-containing amino acid in the fermentation broth from b).

Claims2

Dependent claims

A recombinant microorganism selected from the group consisting of

Corynebacterium glutamicum with increased activity and/or expression of aspartate kinase and attenuation or deletion of the regulator protein McbR compared with the starting strain;
and
Escherichia coli with increased activity and/or expression of aspartate kinase and attenuation or deletion of the regulator protein MetJ compared with the starting strain;
wherein the recombinant microorganism secretes or produces L-methionine, and wherein the recombinant microorganism overexpresses a gene coding for a polypeptide with the activity of a thiosulphate sulphurtransferase comprising the amino acid sequence shown in SEQ ID NO:
2 or an amino acid sequence containing a deletion, substitution, insertion, addition, or combinations thereof, of from 1 to 45 amino acid residues with respect to the amino acid sequence shown in SEQ ID NO:
2, and
wherein the recombinant microorganism has an increased thiosulfate sulfurtransferase activity compared with the starting strain.

4 more elements

Dependencies are inferred from the claim text.