Herbicide tolerant soybean plants and methods for identifying same

A soybean plant, cell, plant part, seed, or progeny thereof, each comprising in its genome elite event EE-GM3, wherein said event comprises in order the following nucleotide sequences:

• (a) the nucleotide sequence of SEQ ID No:
• 2 from nucleotide 1 to 1451;
• (b) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID No:
• 1 from nucleotide 6760 to nucleotide 6958;
• (c) the nucleotide sequence of SEQ ID No:
• 1 from nucleotide 6874 to nucleotide 7298;
• (d) the nucleotide sequence of SEQ ID No:
• 1 from nucleotide 7 to nucleotide 7291;
• (e) the nucleotide sequence of SEQ ID No:
• 1 from nucleotide 12 to nucleotide 7265;
• (f) the nucleotide sequence of SEQ ID No:
• 3 from nucleotide 217 to nucleotide 240;
• (g) the nucleotide sequence of SEQ ID No:
• 3 from nucleotide 241 to nucleotide 1408;
• and further comprising in its genome elite event EE-GM1, wherein said event comprises in order the following sequences:
• (h) the nucleotide sequence of SEQ ID No:
• 12 from nucleotide 1 to 209;
• (i) the nucleotide sequence of SEQ ID No:
• 11 from nucleotide 340 to nucleotide 3461;
• (j) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID No:
• 11 from nucleotide 1 to nucleotide 336;
• (k) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID No:
• 11 from nucleotide 3462 to nucleotide 4076;
• (l) the nucleotide sequence of SEQ ID No:
• 11 from nucleotide 337 to nucleotide 3043;
• (m) the nucleotide sequence of SEQ ID No:
• 13 from nucleotide 559 to nucleotide 568;
• and
• (n) the nucleotide sequence of SEQ ID No:
• 13 from nucleotide 569 to nucleotide 1000.

A plant, cell, plant part, tissue, seed, or progeny thereof, which is

• (a) a soybean plant, cell, plant part, tissue, seed, or progeny thereof, each comprising elite event EE-GM3 and elite event EE-GM2 in its genome, reference seed comprising said elite event EE-GM3 having been deposited at the NCIMB under deposit number NCIMB 41659 and reference seed comprising said elite event EE-GM2 having been deposited at the NCIMB under deposit number NCIMB 41660, or reference seed comprising elite event EE-GM3 and elite event EE-GM2 in its genome having been deposited at the ATCC under deposit number PTA-11042;
• or
• (b) a soybean plant, plant part, tissue, seed or progeny thereof, each comprising elite event EE-GM3 and elite event EE-GM2 in its genome, said soybean plant being obtained by crossing a soybean plant comprising elite event EE-GM3 in its genome, obtainable by propagation of, breeding with, or propagation of and breeding with a soybean plant grown from the seed deposited at the NCIMB under deposit number NCIMB 41659 with a soybean plant comprising elite event EE-GM2 in its genome, obtainable by propagation of, breeding with, or propagation of and breeding with a soybean plant grown from the seed deposited at the NCIMB under deposit number NCIMB 41660, or said soybean plant being obtainable by propagation of, breeding with, or propagation of and breeding with a soybean plant grown from the seed deposited at the ATCC under accession number PTA-11042;
• or
• (c) a soybean seed comprising elite events EE-GM3 and EE-GM2, reference seed comprising elite event EE-GM3 having been deposited at the NCIMB under deposit number NCIMB 41659, reference seed comprising elite event EE-GM2 having been deposited at the NCIMB under deposit number NCIMB 41660, and reference seed comprising elite events EE-GM3 and EE-GM2 having been deposited at the ATCC under deposit number PTA-11042;
• or
• (d) a soybean plant, cell, plant part, tissue, or progeny therof, each comprising elite events EE-GM3 and EE-GM2, produced from the seed of (c);
• or
• (e) a soybean plant, cell, plant part, tissue, seed, or progeny thereof, each comprising elite events EE-GM3 and EE-GM1, wherein said events comprise a nucleic acid sequence of SEQ ID No:
• 2 from nucleotide 1431 to 1472 and a nucleic acid sequence of SEQ ID No:
• 3 from nucleotide 220 to 261, or the complement of said sequences, and also a nucleic acid sequence of SEQ ID No:
• 12 from nucleotide 199 to 220 and a nucleic acid sequence of SEQ ID No:
• 13 from nucleotide 558 to 579, or the complement of said sequences;
• or
• (f) a soybean plant, cell, plant part, tissue, seed, or progeny thereof, each comprising elite events EE-GM3 and EE-GM2, wherein said events comprise a nucleic acid sequence of SEQ ID No:
• 2 from nucleotide 1431 to 1472 and a nucleic acid sequence of SEQ ID No:
• 3 from nucleotide 220 to 261, or the complement of said sequences, and also a nucleic acid sequence of SEQ ID No:
• 14 from nucleotide 301 to 322 and a nucleic acid sequence of SEQ ID No:
• 15 from nucleotide 497 to 518, or the complement of said sequences;
• or
• (g) a soybean plant, cell, plant part, tissue, seed, or progeny therof, each comprising in their genome a nucleic acid molecule comprising a nucleotide sequence of SEQ ID No:
• 20 from nucleotide position 1452 to nucleotide position 16638 or the complement thereof, or a nucleotide sequence of SEQ ID No:
• 20 or the complement thereof, and comprising in their genome a nucleic acid molecule comprising a nucleotide sequence of elite events EE-GM1 or EE-GM2 or the complement thereof, reference seed comprising elite event EE-GM1 having been deposited under deposit number NCIMB 41658, and reference seed comprising elite event EE-GM2 having been deposited under deposit number NCIMB 41660, wherein said nucleotide sequence of elite events EE-GM1 or EE-GM2 comprises or is the foreign DNA sequence in SEQ ID No:
• 12 or 13, or the foreign DNA sequence in SEQ ID No:
• 14 or 15, respectively, or the foreign DNA sequence in the plant genome between the sequence of SEQ ID No:
• 12 and that of SEQ ID No:
• 13, or the foreign DNA sequence in the plant genome between the sequence of SEQ ID No:
• 14 and that of SEQ ID No:
• 15, respectively;
• or
• (h) a soybean plant, cell, plant part, tissue, seed, or progeny thereof, each comprising elite events EE-GM3 and EE-GM2 in its genome, wherein the inserted foreign DNA of elite event EE-GM3 is oriented as indicated in FIG. 1, and wherein said event has 5′ and 3′ flanking DNA and foreign DNA contiguous therewith, wherein said 5′ flanking DNA and the foreign DNA contiguous therewith comprises a nucleotide sequence of SEQ ID No:
• 2, and said 3′ flanking DNA and the foreign DNA contiguous therewith comprises a nucleotide sequence of SEQ ID No:
• 3, said inserted foreign DNA of FIG. 1 being present in the soybean elite event EE-GM3 deposited under deposit number NCIMB 41659, and wherein the inserted foreign DNA of elite event EE-GM2 is oriented as indicated in FIG. 4, and wherein said event has 5′ and 3′ flanking DNA and foreign DNA contiguous therewith, wherein said 5′ flanking DNA and the foreign DNA contiguous therewith comprises a nucleotide sequence of SEQ ID No:
• 14 and said 3′ flanking DNA and the foreign DNA contiguous therewith comprises a nucleotide sequence of SEQ ID No:
• 15, said inserted foreign DNA of FIG. 4 being present in the soybean elite event EE-GM2 deposited under deposit number NCIMB 41660, or
• (i) a soybean plant, cell, plant part, tissue, seed, or progeny thereof, each comprising in their genome a nucleic acid molecule comprising a nucleotide sequence of SEQ ID No:
• 1 from nucleotide position 188 to nucleotide position 7252 or the complement thereof, and comprising in their genome a nucleic acid molecule comprising a nucleotide sequence of elite event EE-GM2 or the complement thereof, reference seed comprising elite event EE-GM2 having been deposited under deposit number NCIMB 41660, wherein said nucleotide sequence of elite event EE-GM2 comprises or is the foreign DNA sequence in SEQ ID No:
• 14 or 15, or the foreign DNA sequence in the plant genome between the sequence of SEQ ID No:
• 14 and that of SEQ ID No:
• 15.

A soybean plant, cell, plant part, seed, or progeny thereof, each comprising elite event EE-GM3, wherein said event comprises in order the following nucleotide sequences:

• (a) the nucleotide sequence of SEQ ID No:
• 2 from nucleotide 1 to 1451;
• (b) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID No:
• 1 from nucleotide 6760 to nucleotide 6958;
• (c) the nucleotide sequence of SEQ ID No:
• 1 from nucleotide 6874 to nucleotide 7298;
• (d) the nucleotide sequence of SEQ ID No:
• 1 from nucleotide 7 to nucleotide 7291;
• (e) the nucleotide sequence of SEQ ID No:
• 1 from nucleotide 12 to nucleotide 7265;
• (f) the nucleotide sequence of SEQ ID No:
• 3 from nucleotide 217 to nucleotide 240;
• and
• (g) the nucleotide sequence of SEQ ID No:
• 3 from nucleotide 241 to nucleotide 1408, and further comprising in its genome elite event EE-GM2, wherein said event comprises in order the following sequences:
• (h) the nucleotide sequence of SEQ ID No:
• 14 from nucleotide 1 to 311;
• (i) the nucleotide sequence of SEQ ID No:
• 11 from nucleotide 3458 to nucleotide 3848;
• (j) the nucleotide sequence of SEQ ID No:
• 11 from nucleotide 413 to nucleotide 3457;
• (k) the nucleotide sequence of SEQ ID No:
• 15 from nucleotide 508 to nucleotide 1880.