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AssigneesKyushu University, National University Corporation, Univeristy of Miyazaki, Konan Gakuen, Nippon Suisan Kaisha, Ltd.
InventorsKeishi Sakaguchi, Rie Hamaguchi, Takanori Matsuda, Makoto Ito, Naoki Nagano, Masahiro Hayashi, Daisuke Honda, Yuji Okita, Shinichi Sugimoto
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To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid.
A method for transforming Stramenopile, the method comprising disrupting a stramenopile gene and/or inhibiting expression thereof by genetic engineering in microorganisms belong to Stramenopile, wherein the microorganisms are selected from the group consisting of Parietichvtrium sarkarianum SEK 364 (FERM BP-11298), Parietichvtrium sp. SEK358 (FERM BP-11405), Parietichvtrium sp. SEK571 (FERM BP-11406), and Schizochvtrium sp. TY 12Ab (FERM BP-11421).
A method for modifying the fatty acid composition of a Stramenopile, the method comprising disrupting a stramenopile gene and/or inhibiting expression thereof by genetic engineering in microorganisms wherein the microorganisms are selected from the group consisting of Parietichvtrium sarkarianum SEK 364 (FERM BP-11298), Parietichvtrium sp. SEK358 (FERM BP-11405), Parietichvtrium sp. SEK571 (FERM BP-11406), and Schizochvtrium sp. TY 12Ab (FERM BP-11421).
A stramenopile transformed for the modification of the fatty acid composition through disruption of its gene and/or inhibition of expression thereof by genetic engineering in microorganisms wherein the microorganisms are selected from the group consisting of Parietichvtrium sarkarianum SEK 364 (FERM BP-11298), Parietichvtrium sp. SEK358 (FERM BP-11405), Parietichvtrium sp. SEK571 (FERM BP-11406), and Schizochvtrium sp. TY 12Ab (FERM BP-11421).
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