Detecting a high molecular weight substance
AssigneeCharm Sciences, Inc.
InventorSteven J. Saul
Life of this patent
Estimating the term…
Abstract
Label complexes, lateral flow apparatus and methods of detecting a high molecular weight substance are shown and described. In one embodiment, the label complex includes an antispecies antibody and another antibody having sensitivity to both the antispecies antibody and to a control line capture agent, an antibody binding protein and a detectable component. Typically, the antibody binding protein may bind to a receptor for the analyte or to the anti-species antibody. In yet other embodiments, a lateral flow test strip includes the label complex and a solid support that traverses lateral flow of a liquid sample and provides a detectable signal.
Claims11
A lateral flow test strip for the analysis of one or more analytes in a liquid sample, the test strip comprising:
- a) a label complex, the label complex including:
- (i) at least two antibodies, one of the at least two antibodies including an antispecies antibody and another of the at least two antibodies including an antibody having a sensitivity to the antispecies antibody and to a control line capture agent, the control line capture agent being bound to a control line and characterized in that the control line capture agent has affinity to the label complex independent of the label complex being bound by the one or more analytes;
- (ii) at least one bound antibody binding protein having affinity to the anti-species antibody;
- (iii) at least one free antibody binding protein adapted to bind to at least one receptor for the one or more analytes;
- and
- (iv) a detectable component being bound to the at least one bound antibody binding protein and to the at least one free antibody binding protein;
- and
- b) a solid support, the solid support supporting the label complex, the control line and at least one test line having a test line capture agent immobilized thereon, wherein the solid support comprises a body adapted to traverse lateral flow and a surface to provide a detectable signal,
- whereby the detectable signal generates an intensity when the at least one analyte receptor is captured by either the control line capture agent or the test line capture agent.
Dependencies are inferred from the claim text.