Direct conversion of cells to cells of other lineages
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsMarius Wernig, Thomas C. Sudhof, Thomas Vierbuchen, Austin Ostermeier, Zhiping Pang
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Abstract
Methods, compositions and kits for producing functional neurons, astroctyes, oligodendrocytes and progenitor cells thereof are provided. These methods, compositions and kits find use in producing neurons, astrocytes, oligodendrocytes, and progenitor cells thereof for transplantation, for experimental evaluation, as a source of lineage- and cell-specific products, and the like, for example for use in treating human disorders of the CNS. Also provided are methods, compositions and kits for screening candidate agents for activity in converting cells into neuronal cells, astrocytes, oligodendrocytes, and progenitor cells thereof.
Claims20
A method of converting non-neuronal cells into induced neuronal/oligodendrocytes/astrocytes/neural stem cells (iNs), the method comprising:
- contacting a population of non-neuronal somatic cells in vitro with a neuron reprogramming (NR) system consisting of reprogramming factors Asci1, Brn2 and Myt1I for a period of time sufficient to reprogram said non-neural cells, wherein a population of iNs is produced.
A method of converting fibroblast cells into induced neuronal/oligodendrocytes/astrocytes/neural stem cells (iNs), the method comprising:
- contacting a population of fibroblast cells in vitro with a neuron reprogramming (NR) system consisting of reprogramming factors Asci1, Brn2, Myt1I, for a period of time sufficient to reprogram said fibroblast cells,
- wherein a population of iNs is produced.
A method of converting fibroblast cells into induced neuronal/oligodendrocytes/astrocytes/neural stem cells (iNs), the method comprising:
- contacting a population of human fibroblast cells in vitro with a neuron reprogramming (NR) system consisting of reprogramming factors Asci1, Brn2, Myt1I and NeuroD, for a period of time sufficient to reprogram said fibroblast cells, wherein a population of iNs is produced.
Dependencies are inferred from the claim text.