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US 9,051,591 B2Granted
Bacterium of enterobacteriaceae family producing L-aspartic acid or L-aspartic acid-derived metabolites and a method for producing L-aspartic acid or L-aspartic acid-derived metabolites
AssigneeAjinomoto Co., Inc.
InventorsTatyana Mikhailovna Kuvaeva, Sergey Vasilievich Smirnov, Olga Nikolaevna Ivanova, Aleksandr Dmitrievich Kivero, Joanna Yosifovna Katashkina
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Abstract
The presently disclosed subject matter provides a bacterium of Enterobacteriaceae family producing L-aspartic acid or an L-aspartic acid-derived metabolite modified to have aspartate dehydrogenase and a method for producing L-aspartic acid or an L-aspartic acid-derived metabolite, such as L-threonine, L-lysine, L-arginine, L-methionine and L-homoserine, using such bacterium.
Claims7
A method for producing L-aspartic acid or an L-aspartic acid-derived metabolite comprising:
- cultivating a bacterium belonging to the genus Pantoea or Escherichia, which produces L-aspartic acid or an L-aspartic acid-derived metabolite, in a culture medium to produce and excrete the L-aspartic acid or the L-aspartic acid-derived metabolite into the medium, and collecting L-aspartic acid or said L-aspartic acid-derived metabolite from the medium,
- wherein said bacterium has been modified by introducing a gene encoding aspartate dehydrogenase derived from Bradyrhizobium japonicum wherein the gene encodes an amino acid sequence not less than 95% identical to the amino acid sequence encoded by SEQ ID NO:
- 68, and
- wherein said L-aspartic acid-derived metabolite is selected from the group consisting of L-threonine, L-lysine, L-arginine, L-methionine and L-homoserine.
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Dependencies are inferred from the claim text.