Method for immune cell-assisted bacterial culturing
AssigneeMRIGlobal
InventorsKelly Brown, Richard Winegar, Jacqueline Bortzner, Nicole Moritz, Stephanie Sorrell
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Abstract
A method of culturing fastidious bacteria where a conditioned cell culture medium is prepared in which eukaryotic cells have been cultured, but where the medium is substantially free of the cultured cells. Fastidious bacteria are cultured in the medium. The conditioned cell culture medium can contain secreted factors from the cells. These factors promote the growth of the fastidious bacteria. The fastidious bacterial culture is maintained for a time period sufficient for the fastidious bacteria to multiply. The fastidious bacteria are analyzed using PCR, DNA sequencing or microbiology characterization.
Claims18
A method of culturing fastidious bacteria comprising the steps of:
- a) providing a conditioned cell culture medium in which mammalian macrophages have been cultured that is substantially free of said cultured macrophages wherein a mycotoxin is added to said medium to prevent engulfment of said fastidious bacteria by said culture macrophages;
- b) culturing fastidious bacteria in said medium;
- and
- c) maintaining said fastidious bacterial culture for a time period sufficient for said fastidious bacteria to multiply.
An immune cell-assisted bacterial culturing method comprising the steps of:
- a) obtaining macrophage cells;
- b) propagating said macrophage cells in a medium;
- c) filtering said macrophage cells to remove said macrophage cells from said medium creating a filtered, condition medium containing at least one factor secreted from said macrophage cells and substantially no cells;
- d) introducing said Francisella tularensis to said filtered, conditioned medium;
- e) maintaining said Francisella tularensis in said filtered, condition medium under time, temperature, and atmospheric conditions suitable for culturing said Francisella tularensis;
- and
- f) assaying said Francisella tularensis for analysis.
A method of culturing fastidious bacteria comprising the steps of:
- a) providing a conditioned cell culture medium in which eukaryotic cells have been cultured that is substantially free of said cultured cells and contains at least one factor secreted from said cells;
- b) culturing fastidious bacteria in said medium, wherein the fastidious bacteria are gram negative;
- and
- c) maintaining said fastidious bacterial culture under time, temperature, and atmospheric conditions suitable for culturing said fastidious bacteria, wherein said at least one factor promotes the growth of said fastidious bacteria;
- and
- d) assaying said fastidious bacteria for analysis.
Dependencies are inferred from the claim text.