PCR method and PCR device
AssigneeFluid Incorporated
InventorShuzo Hirahara
Life of this patent
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Abstract
Provided on an inner surface of a container to carry out polymerase chain reaction (PCR) are an electrode pair 22 to be disposed to face each other with a gap along a flow of a reaction solution interposed therebetween, and an AC voltage is applied to the electrode pair 22 to make an AC current flow through the reaction solution, whereby Joule heat is generated to control the reaction solution in temperature. Accordingly, a PCR method and a PCR device that allow generating sufficient Joule heat for a PCR cycle even when an applied voltage is low, and do not electrolyze a reaction solution even by making an electric current flow through the reaction solution can be provided.
Claims15
A polymerase chain reaction (PCR) method comprising the steps of:
- providing, on an inner surface of a container to carry out polymerase chain reaction (PCR), an electrode pair to be disposed to face each other with a gap as part of a flowpath of a reaction solution interposed therebetween;
- applying an AC voltage to the electrode pair to make an AC current flow through the reaction solution, thereby generating Joule heat to control the reaction solution in temperature;
- flowing the reaction solution intermittently through the gap while the entire reaction solution within the container is controlled in temperature so as to be a complete PCR cycle;
- and
- performing a PCR cycle in the container.
A polymerase chain reaction (PCR) method comprising the steps of:
- providing, on an inner surface of a container to carry out polymerase chain reaction (PCR), an electrode pair to be disposed to face each other with a gap as part of a flowpath of a reaction solution interposed therebetween;
- applying an AC voltage to the electrode pair to make an AC current flow through the reaction solution, thereby generating Joule heat to control the reaction solution in temperature;
- arresting the reaction solution in the container such that the entire reaction solution is controlled in temperature so as to be a PCR cycle;
- and
- performing a PCR cycle in the container.
A polymerase chain reaction (PCR) method comprising the steps of:
- providing, on an inner surface of a container to carry out polymerase chain reaction (PCR), an electrode pair to be disposed to face each other with a gap as part of a flowpath of a reaction solution interposed therebetween;
- applying an AC voltage to the electrode pair to make an AC current flow through the reaction solution, thereby generating Joule heat to control the reaction solution in temperature;
- and
- performing a PCR cycle in the container,
- wherein the electrode pair creates an electric field applied in a perpendicular direction compared to a direction of the flowpath.
Dependencies are inferred from the claim text.