Method for producing a target substance by fermentation
AssigneeAjinomoto Co., Inc.
InventorsYousuke Nishio, Youko Yamamoto, Kazuteru Yamada, Kosuke Yokota
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Abstract
A target substance can be produced by culturing a bacterium having an ability to produce 2-ketoglutaric acid or a derivative thereof, and an ability to produce xylonic acid from xylose, which is imparted with xylonate dehydratase activity, 2-keto-3-deoxyxylonate dehydratase activity and 2-ketoglutaric semialdehyde dehydrogenase activity, or in which these activities are enhanced, in a medium containing xylose as a carbon source to produce and accumulate the target substance in the medium, and collecting the target substance from the medium.
Claims6
A method for producing a target substance comprising
- A) culturing a bacterium belonging to the genus Pantoea or the genus Corynebacterium and having an ability to produce the target substance in a medium containing xylose wherein the bacterium is able to produce the target substance through conversion of xylonic acid produced from xylose into 2-ketoglutaric acid by xylonate dehydratase, 2-keto-3-deoxyxylonate dehydratase, and 2-ketoglutaric semialdehyde dehydrogenase,
- B) allowing the bacterium to produce and secrete the target substance into the medium, and
- C) collecting the target substance from the medium,
- wherein the target substance is 2-ketoglutaric acid or a derivative thereof, the bacterium has an ability to produce xylonic acid from xylose, and activities of the enzymes xylonate dehydratase, 2-keto-3-deoxyxylonate dehydratase, and 2-ketoglutaric semialdehyde dehydrogenase have been imparted to or enhanced in the bacterium;
- wherein the 2-ketoglutaric acid derivative is selected from the group consisting of L-glutamic acid, L-glutamine, L-arginine, L-citrulline, L-ornithine, L-proline, putrescine, and γ-aminobutyric acid,
- wherein the bacterium can produce xylonic acid from xylose because of any one of the following characteristics:
- (i) xylose dehydrogenase activity, or xylose dehydrogenase activity and xylonolactonase activity have been imparted to or enhanced in the bacterium, or
- (ii) the bacterium has glucose dehydrogenase activity that can catalyze a reaction producing xylonic acid from xylose,
- wherein said activities are imparted to or enhanced in the bacterium by introducing expressable forms of genes coding for the enzymes into the bacterium, wherein the genes are native to a microorganism belonging to a genus selected from the group consisting of Caulobacter, Escherichia, Agrobacterium, Herbaspirillum, Actinoplanes, Cupriavidus, Pseudomonas, Zobellia, Thermobacillus, Arthrobacter, Azospirillum, Halomonas, Bacillus, and Aspergillus.
Dependencies are inferred from the claim text.