Polypeptides having oxidoreductase activity and their uses
AssigneePurac Biochem B.V.
InventorsHarald Johan Ruijssenaars, Nick Johannes Petrus Wierckx, Frank Wouter Koopman, Adrianus Johannes Jozef Straathof, Johannes Hendrik De Winde
Life of this patent
Estimating the term…
Abstract
The invention relates to a polypeptide having oxidoreductase activity which comprises the amino acid sequence set out in SEQ ID NO: 3 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 4, or a variant polypeptide thereof having 45% or more sequence identity with the sequence of SEQ ID NO: 3. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) or production of 5-hydroxymethyl-2-furancarboxylic acid (HMF acid).
Claims14
An isolated cell comprising an expression construct for expression of a heterologous nucleotide sequence encoding an oxidoreductase having an amino acid sequence with at least 95% identity with the amino acid sequence of SEQ ID NO:
- 3, wherein, said expression construct is expressible in said cell and expression thereof of said oxidoreductase confers to or increases in the cell the ability to oxidize at least one of 5-hydroxymethylfurfural (HMF) and 5-hydroxymethyl-2-furancarboxylic acid (HMF acid) to 2,5-furandicarboxylic acid (FDCA), as compared to a corresponding wild type cell lacking the expression construct.
An expression construct for expression of a heterologous nucleotide sequence encoding an oxidoreductase in a cell, wherein the oxidoreductase has an amino acid sequence with at least 95% identity with the amino acid sequence of SEQ ID NO:
- 3, wherein, said expression construct when said oxidoreductase is expressed thereof in said cell confers to or increases in the cell the ability to oxidize at least one of 5-hydroxymethylfurfural (HMF) and 5-hydroxymethyl-2-furancarboxylic acid (HMF acid) to 2,5-furandicarboxylic acid (FDCA), and wherein at least one regulatory sequence that is necessary for expression of the nucleotide sequence encoding said oxidoreductase and operably linked to said nucleotide sequence, is foreign to said nucleotide sequence.
Dependencies are inferred from the claim text.