Oncolytic rhabdovirus
AssigneesOttawa Hospital Research Institute, Children's Hospital of Eastern Ontario Research Institute Inc.
InventorsJohn C. Bell, David F. Stojdl
Life of this patent
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Abstract
Embodiments of the invention include compositions and methods related to Maraba virus and their use as anti-cancer therapeutics. Such rhabdoviruses possess tumor cell killing properties in vitro and in vivo.
Claims32
An attenuated rhabdovirus comprising:
- an M protein having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:4 and a tryptophan at the position corresponding to position 123 of SEQ ID NO:4, or
- both a G protein having an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:
- 5 and an arginine at the position corresponding to position 242 of SEQ ID NO:5 and an M protein having an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:4 and a tryptophan at the position corresponding to position 123 of SEQ ID NO:4.
A method of killing a hyperproliferative cell comprising contacting the cell with an isolated oncolytic rhabdovirus encoding:
- an M protein having an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:4 and a tryptophan at the position corresponding to position 123 of SEQ ID NO:4, or
- both a G protein having an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:
- 5 and an arginine at the position corresponding to position 242 of SEQ ID NO:5 and an M protein having an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:4 and a tryptophan at the position corresponding to position 123 of SEQ ID NO:4.
A method for treating a cancer patient comprising administering an effective amount of an isolated oncolytic rhabdovirus encoding:
- an M protein having an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:4 and a tryptophan at the position corresponding to position 123 of SEQ ID NO:4, or
- both a G protein having an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:
- 5 and an arginine at the position corresponding to position 242 of SEQ ID NO:5 and an M protein having an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:4 and a tryptophan at the position corresponding to position 123 of SEQ ID NO:4.
A composition comprising an isolated oncolytic rhabdovirus having a nucleic acid segment encoding:
- an M protein having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:4 and a tryptophan at the position corresponding to position 123 of SEQ ID NO:4, or
- both a G protein having an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:
- 5 and an arginine at the position corresponding to position 242 of SEQ ID NO:5 and an M protein having an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:4 and a tryptophan at the position corresponding to position 123 of SEQ ID NO:4.
Dependencies are inferred from the claim text.