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US 9,045,728 B2Granted
Liquid viral formulations
AssigneeOncolytics Biotech Inc.
InventorsMatthew C. Coffey, Sarah Serl, Leo Pavliv
Life of this patent
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Abstract
Provided herein are liquid viral formulations useful for the stabilization and storage of viruses and methods of preparing these formulations. The liquid viral formulations described herein include a virus (e.g., a purified virus) and a non-viral composition including excipients and a liquid carrier. The formulations can be used, for example, to retain the infectivity or immunogenicity of viruses during periods of storage.
Claims35
A viral formulation comprising:
- (a) a purified virus;
- and
- (b) a non-viral composition comprising:
- (i) mannitol;
- (ii) sorbitol in a concentration of less than 2.5% based on the weight of the non-viral composition;
- (iii) histidine;
- (iv) Mg2+;
- and
- (v) a liquid carrier,
- wherein the non-viral composition, excluding the liquid carrier, is free of monovalent cationic salts and wherein the non-viral composition is provided in an amount effective to stabilize the purified virus.
7 more elements
A method of making a viral formulation, comprising the steps of:
- (a) providing a virus;
- and
- (b) combining the virus with a non-viral composition comprising:
- (i) mannitol;
- (ii) sorbitol in a concentration of less than 2.5% based on the weight of the non-viral composition;
- (iii) histidine;
- (iv) Mg2+;
- and
- (v) a liquid carrier,
- wherein the non-viral composition, excluding the liquid carrier, is free of monovalent cationic salts, and wherein the non-viral composition is provided in an amount effective to stabilize the purified virus to form a viral formulation.
7 more elements
A non-viral composition for use in preserving or stabilizing a virus comprising:
- (a) mannitol;
- (b) sorbitol in a concentration of less than 2.5% based on the weight of the non-viral composition;
- (c) histidine;
- (d) Mg2+;
- and
- (e) a liquid carrier,
- wherein the non-viral composition, excluding the liquid carrier, is free of monovalent cationic salts, and wherein the non-viral composition is provided in an amount effective to stabilize said virus.
4 more elements
Dependencies are inferred from the claim text.