# US 9,040,792 B2 · Osteospermum and Dimorphoteca plants having an altered flower phenotype > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,040,792 B2 - Document ID: US9040792B2 - Status: Granted - Kind: B2 - Application number: 12/750,965 - Filed: 2010-03-31 - Published: 2011-10-06 - Granted: 2015-05-26 - Earliest claimed: 2010-03-31 - Assignees: Klemm+Sohn GmbH & Co. KG - Applicants: Andrea Dohm; Ulrich Sander; Nils Klemm - Inventors: Andrea Dohm; Ulrich Sander; Nils Klemm - Web page: https://patentgrep.com/patent/US9040792B2 ## CPC - A01H 6/14: Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce - A01H 5/02: Flowers - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ## Abstract The present invention relates to an Osteospermum and Dimorphoteca plant, seed, variety, and hybrid. Another aspect of the present invention relates to an Osteospermum and Dimorphoteca plant having a mutant allele designated KLEDF which results in an altered flower phenotype. The invention also relates to crossing Osteospermum and Dimorphoteca plants containing the KLEDF mutant allele with other Osteospermum and Dimorphoteca plants lacking the KLEDF mutant allele to produce intergeneric and interspecific hybrids. This invention further relates to specific lines of Osteospermum varieties exhibiting the altered-flowering phenotype. Furthermore, the invention relates to pollen, seed, and sexual, as well as asexual progeny of such plants with altered flowers. In addition, the invention relates to methods for propagating said plants and to uses of said plants. ## Claims ### Claim 1 An Osteospermum plant comprising at least one dominant mutant allele that produces an altered flower characteristic, wherein said Osteospermum plant having an altered flower has at least one inflorescence which has at least one disc floret with an average length of at least 1.3 cm, and wherein a sample of representative seed of the Osteospermum plant comprising said dominant mutant allele that produces said altered flower is deposited under NCIMB Accession No. 41698. ### Claim 2 (depends on 1) The Osteospermum plant of claim 1, wherein said disc floret ranges from being tubular shaped to a secondary ligulate floret. ### Claim 3 (depends on 1) The Osteospermum plant of claim 1, wherein said at least one disc floret has an average length between 1.21 between 1.3 cm and 2.4 cm. ### Claim 4 (depends on 1) The Osteospermum plant of claim 1, wherein said at least one disc floret has an average length between 2.41 cm and 4.53 cm. ### Claim 5 (depends on 1) The Osteospermum plant of claim 1, wherein said at least one disc floret has an average length between 4.54 cm and 6.0 cm. ### Claim 6 (depends on 1) A method for producing an Osteospermum plant having an altered flower, wherein the method comprises crossing the plant of claim 1 with a different Osteospermum plant not having an altered flower, and selecting for F1 offspring having an altered flower. ### Claim 7 An Osteospermum seed comprising at least one dominant mutant allele that produces an altered flower characteristic designated KLEDF, wherein a representative sample of Osteospermum seed containing said dominant mutant allele that produces an altered flower characteristic was deposited under NCIMB Accession No. 41698. ### Claim 8 (depends on 7) An Osteospermum plant, or a part thereof, produced by growing the seed of claim 7. ### Claim 9 (depends on 1) A tissue culture of regenerable cells produced from the plant of claim 1, wherein said cells of the tissue culture are produced from a plant part selected from the group consisting of leaf, pollen, embryo, cotyledon, hypcotyl, meristematic cell, protoplast, root, root tip, pistil, anther, stem, petiole, ray floret, and disc floret. ### Claim 10 (depends on 9) A plant regenerated from said tissue culture of claim 9, wherein said plant has an altered flower having at least one inflorescence that has at least one disc floret with an average length of at least 1.3 cm. ### Claim 11 (depends on 8) A method for producing Osteospermum seed containing mutant allele KLEDF, wherein the method comprises crossing the plant of claim 8 with a different Osteospermum plant and harvesting the resulting seed. ### Claim 12 (depends on 8) A method for producing an Osteospermum plant having an altered flower conferred by mutant allele KLEDF, wherein the method comprises: a. obtaining the Osteospermum plant of claim 8; b. transferring the KLEDF mutant allele from said Osteospermum plant to an Osteospermum plant not having an altered flower, wherein said transfer is performed via a transgenic method; and c. selecting for recipient Osteospermum plants containing the KLEDF mutant allele. ### Claim 13 (depends on 12) The method of claim 12, wherein said transgenic transfer of the KLEDF mutant allele is performed into a protoplast or cell culture. ### Claim 14 (depends on 12) A plant produced by the method of claim 12, wherein said plant has the KLEDF allele. ### Claim 15 (depends on 14) A cell or protoplast produced from the plant of claim 14. ### Claim 16 (depends on 15) The cell or protoplast of claim 15, wherein said cell or protoplast is transgenic. ### Claim 17 (depends on 11) A plant produced from the seed of claim 11, wherein said plant has an altered flower conferred by mutant allele KLEDF. ### Claim 18 (depends on 17) A method for transferring mutant allele KLEDF to a different genetic background, wherein the method comprises: a. obtaining the the plant of claim 17; b. backcrossing said plant to a recipient parent plant not having an altered flower to produce backcross progeny plants; c. selecting for backcross progeny plants that contain mutant allele KLEDF; d. backcrossing said backcross progeny plants to said recipient parent; e. repeating steps (c) and (d) two or more times in succession to produce selected third or higher backcross progeny plants that contain mutant allele KLEDF; and f. harvesting the resulting seed. ### Claim 19 (depends on 18) A plant produced from the seed of claim 18. ### Claim 20 (depends on 11) An Osteospermum seed containing mutant allele KLEDF produced by the method of claim 11. ### Claim 21 (depends on 1) A method for transferring an altered flower trait to a different genetic background, wherein the method comprises: a. obtaining the plant of claim 1; b. backcrossing said plant to a recipient parent plant not having an altered flower to produce backcross progeny plants; c. selecting for backcross progeny plants to said recipient parent; d. repeating steps (c) and (d) two or more times in succession to produce selected third or higher backcross progeny plants that have an altered flower; and e. harvesting the resulting seed. ### Claim 22 (depends on 21) A plant grown from the seed produced by the method of claim 21, wherein said plant comprises the dominant mutant allele and has an altered flower. ### Claim 23 (depends on 1) The Osteospermum plant of claim 1 having at least one inflorescence that has a disc floret length that is 73% or greater of the length of the ray floret. ### Claim 24 (depends on 1) The Osteospermum plant of claim 1 having at least one inflorescence that has a disc floret length that is 67% or greater of the length of the ray floret. ### Claim 25 (depends on 1) The Osteospermum plant of claim 1 having at least one inflorescence that has a disc floret length that is 50% or greater of the length of the ray floret. ### Claim 26 (depends on 1) The Osteospermum plant of claim 1 having at least one inflorescence that has a disc floret length that is 44% or greater of the length of the ray floret. ### Claim 27 (depends on 1) The Osteospermum plant of claim 1 having at least one inflorescence that has a disc floret length that is 38.5% or greater of the length of the ray floret. ### Claim 28 (depends on 1) The Osteospermum plant of claim 1 having at least one inflorescence that has a disc floret length that ranges from 38.5% to 43% of the length of the ray floret. ### Claim 29 (depends on 1) The Osteospermum plant of claim 1 having at least one inflorescence that has a disc floret length that ranges from 43% to 50% of the length of the ray floret. ### Claim 30 (depends on 1) The Osteospermum plant of claim 1 having at least one inflorescence that has a disc floret length that ranges from 50% to 67% of the length of the ray floret. ### Claim 31 (depends on 1) The Osteospermum plant of claim 1 having at least one inflorescence that has a disc floret length that ranges from 67% to 73% of the length of the ray floret. ### Claim 32 (depends on 1) The Osteospermum plant of claim 1 having at least one inflorescence that has a disc floret length that ranges from 73% to 80% of the length of the ray floret.