# US 9,040,783 B2 · Barley cultivar BG-161

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- Number: US 9,040,783 B2
- Document ID: US9040783B2
- Status: Granted
- Kind: B2
- Application number: 13/644,224
- Filed: 2012-10-03
- Published: 2014-04-03
- Granted: 2015-05-26
- Earliest claimed: 2012-10-03
- Assignees: Monsanto Technology LLC
- Applicants: Monsanto Technology LLC
- Inventors: Dale R. Clark
- Web page: https://patentgrep.com/patent/US9040783B2

## CPC

- C12N 15/8289: {Male sterility}
- A01H 5/10: Seeds
- C12N 15/8245: {involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis}
- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C12N 15/8251: {Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis}
- C12N 15/8273: {for drought, cold, salt resistance}
- C12N 15/8274: {for herbicide resistance}
- C12N 15/8275: {Glyphosate}
- C12N 15/8278: {Sulfonylurea}
- C12N 15/8282: {for fungal resistance}
- C12N 15/8283: {for virus resistance}
- C12N 15/8286: {for insect resistance}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

A barley cultivar, designated BG-161, is disclosed. The invention relates to seeds, plants, and hybrids of barley cultivar BG-161, and methods for producing a barley plant produced by crossing plants from barley cultivar BG-161 with themselves or plants from another barley variety. The invention also relates to methods for producing a barley plant containing in its genetic material one or more transgenes and to the transgenic barley plants and plant parts produced by those methods. The invention also relates to barley varieties derived from barley cultivar BG-161, to methods for producing other barley varieties, lines or plant parts derived from barley cultivar BG-161, and to the barley plants, varieties, and their parts derived from the use of those methods. The invention further relates to hybrid barley seeds and plants produced by crossing barley cultivar BG-161 with another barley cultivar.

## Claims

### Claim 1

A seed of barley cultivar BG-161, a representative sample of seed of which was deposited under ATCC Accession No. PTA-121758.

### Claim 2 (depends on 1)

A barley plant, or a part thereof, produced by growing the seed of claim 1.

### Claim 3 (depends on 2)

A tissue culture produced from protoplasts or cells from the plant of claim 2, wherein said protoplasts or cells of the tissue culture are produced from a plant part selected from the group consisting of head, awn, leaf, pollen, embryo, cotyledon, hypocotyl, seed, spike, pericarp, meristematic cell, root, root tip, pistil, anther, floret, shoot, stem and callus.

### Claim 4 (depends on 3)

A barley plant regenerated from the tissue culture of claim 3, wherein the plant has essentially all of the morphological and physiological characteristics of barley cultivar BG-161.

### Claim 5 (depends on 2)

A method for producing a barley seed comprising crossing two barley plants and harvesting the resultant barley seed, wherein at least one barley plant is the barley plant of claim 2.

### Claim 6 (depends on 5)

A barley seed produced by the method of claim 5.

### Claim 7 (depends on 6)

A barley plant, or a part thereof, produced by growing said seed of claim 6.

### Claim 8 (depends on 2)

A method of producing an herbicide tolerant barley plant wherein the method comprises transforming the barley plant of claim 2 with a transgene wherein the transgene confers tolerance to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, phenoxy proprionic acid, cyclohexane, L-phosphinothricin, triazine and benzonitrile.

### Claim 9 (depends on 8)

An herbicide tolerant barley plant produced by the method of claim 8.

### Claim 10 (depends on 2)

A method of producing a pest or insect resistant barley plant wherein the method comprises transforming the barley plant of claim 2 with a transgene that confers pest or insect resistance.

### Claim 11 (depends on 10)

A pest or insect resistant barley plant produced by the method of claim 10.

### Claim 12 (depends on 11)

The barley plant of claim 11, wherein the transgene encodes a Bacillus thuringiensis endotoxin.

### Claim 13 (depends on 2)

A method of producing a disease resistant barley plant wherein the method comprises transforming the barley plant of claim 2 with a transgene that confers disease resistance.

### Claim 14 (depends on 13)

A disease resistant barley plant produced by the method of claim 13.

### Claim 15 (depends on 2)

A method of producing a barley plant with modified fatty acid metabolism, modified carbohydrate metabolism or modified protein metabolism wherein the method comprises transforming the barley plant of claim 2 with a transgene encoding a protein selected from the group consisting of modified glutenins, gliadins, phytase, lipoxygenase, beta-glucanase, polyphenol oxidase, fructosyltransferase, levansucrase, α-amylase, invertase and starch branching enzyme or encoding an antisense of stearyl-ACP desaturase.

### Claim 16 (depends on 15)

A barley plant having modified fatty acid metabolism, modified carbohydrate metabolism or modified protein metabolism produced by the method of claim 15.

### Claim 17

A method of introducing a desired trait into barley cultivar BG-161 wherein the method comprises:
(a) crossing a BG-161 plant, wherein a representative sample of seed was deposited under ATCC Accession No. PTA-121758, with a plant of another barley cultivar that comprises a desired trait to produce progeny plants wherein the desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance, modified fatty acid metabolism, modified carbohydrate metabolism, modified phytic acid metabolism, modified waxy starch content, modified protein content, increased stress to water tolerance and resistance to bacterial disease, fungal disease or viral disease;
(b) selecting one or more progeny plants that have the desired trait to produce selected progeny plants;
(c) crossing the selected progeny plants with the BG-161 plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait and essentially all of the physiological and morphological characteristics of barley cultivar BG-161 listed in Table 1 to produce selected backcross progeny plants; and
(e) repeating the crossing the selected progeny step and selecting for backcross progeny step two or more times in succession to produce selected third or higher backcross progeny plants that comprise the desired trait and essentially all of the physiological and morphological characteristics of barley cultivar BG-161 listed in Table 1.

### Claim 18 (depends on 17)

A barley plant produced by the method of claim 17, wherein the plant has the desired trait.

### Claim 19 (depends on 18)

The barley plant of claim 18, wherein the desired trait is herbicide tolerance and the tolerance is conferred to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, phenoxy proprionic acid, cyclohexane, L-phosphinothricin, triazine and benzonitrile.

### Claim 20 (depends on 18)

The barley plant of claim 18, wherein the desired trait is insect resistance and the insect resistance is conferred by a transgene encoding a Bacillus thuringiensis endotoxin.

### Claim 21 (depends on 18)

The barley plant of claim 18, wherein the desired trait is modified fatty acid metabolism, modified carbohydrate metabolism or modified protein metabolism and said desired trait is conferred by a nucleic acid encoding a protein selected from the group consisting of modified glutenins, gliadins, phytase, lipoxygenase, beta-glucanase, polyphenol oxidase, fructosyltransferase, levansucrase, α-amylase, invertase and starch branching enzyme or encoding an antisense of stearyl-ACP desaturase.

### Claim 22 (depends on 20)

The barley plant of claim 20, wherein the desired trait is male sterility and the trait is conferred by a nucleic acid molecule that confers male sterility.