# US 9,040,772 B2 · Compositions and methods for enhancing resistance to northern leaf blight in maize

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- Number: US 9,040,772 B2
- Document ID: US9040772B2
- Status: Granted
- Kind: B2
- Application number: 13/696,153
- Filed: 2011-06-24
- Published: 2013-03-07
- Granted: 2015-05-26
- Earliest claimed: 2010-06-25
- Assignees: E. I. Du Pont de Nemours and Company
- Applicants: Bailin Li; William A. Wilson
- Inventors: Bailin Li; William A. Wilson
- Web page: https://patentgrep.com/patent/US9040772B2

## CPC

- C12N 15/8282: {for fungal resistance}
- A01H 1/04: Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection}
- A01H 1/045: {using molecular markers}
- A01H 5/10: Seeds
- A01H 6/4684: {Zea mays [maize]}
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- C12Q 2600/172: Haplotypes
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The invention relates to methods and compositions for identifying and selecting maize plants with enhanced resistance to northern leaf blight. Maize plants generated by the methods of the invention are also a feature of the invention.

## Claims

### Claim 1

A method of selecting a maize plant with enhanced resistance to northern leaf blight, the method comprising:
a. isolating nucleic acids from a maize plant;
b. analyzing the isolated nucleic acids for the presence of a QTL allele associated with the enhanced resistance to northern leaf blight, wherein the presence of said QTL allele is determined by detecting in the maize plant at least one marker allele that is linked to and associated with a haplotype selected from the group consisting of:
i. a “G” at PHM13395-27 and an “A” at PHM3418-12; and
ii. a “C” at PHM2817-26 and an “A” at PHM7446-6; and wherein said at least one marker allele is within 10 cM of the haplotype; and
b. selecting said maize plant that has the at least one marker allele.

### Claim 2 (depends on 1)

The method of claim 1, wherein the at least one marker allele is within 5 cM of the haplotype.

### Claim 3 (depends on 1)

The method of claim 1, wherein the at least one marker allele is within 1 cM of the haplotype.

### Claim 4

A method of selecting a maize plant with enhanced resistance to northern leaf blight, the method comprising:
a. isolating nucleic acids from a maize plant;
b. analyzing the isolated nucleic acids for the presence of a QTL allele associated with the enhanced resistance to northern leaf blight, wherein the presence of said QTL allele is determined by detecting in the maize plant a haplotype selected from the group consisting of:
i. a “G” at PHM13395-27 and an “A” at PHM3418-12; and
ii. a “C” at PHM2817-26 and an “A” at PHM7446-6; and
b. selecting said maize plant that has said haplotype.