# US 9,040,275 B2 · Laccase for detoxification of feed products

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- Number: US 9,040,275 B2
- Document ID: US9040275B2
- Status: Granted
- Kind: B2
- Application number: 12/333,414
- Filed: 2008-12-12
- Published: 2009-06-18
- Granted: 2015-05-26
- Earliest claimed: 2007-12-14
- Assignees: Novozymes A/S
- Applicants: Anders Vikso-Nielsen; Birthe Hauerbach Sorensen
- Inventors: Anders Vikso-Nielsen; Birthe Hauerbach Sorensen
- Web page: https://patentgrep.com/patent/US9040275B2

## CPC

- C12P 1/00: Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- A23K 10/14: Pretreatment of feeding-stuffs with enzymes
- A23K 10/38: from distillers' or brewers' waste
- A23K 50/30: for swines
- A23L 5/25: {using enzymes}
- C12N 9/0061: {Laccase (1.10.3.2)}
- Y02P 60/87: Re-use of by-products of food processing for fodder production
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- A23K: FODDER
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

## Abstract

The present invention relates to a method for detoxification of feed products contaminated by the mycotoxin zearalenone.

## Claims

### Claim 1

A process for degrading zearalenone comprising treating the zearalenone with a laccase.

### Claim 2 (depends on 1)

The process of claim 1, wherein the zearalenone is dihydroxy-4-methyl-3-oxabicyclo[12.4.0]octadeca-12,15,17,19-tetraene-2,8-dione.

### Claim 3 (depends on 1)

The process of claim 1, wherein the dosage of the laccase is 0.01-100 mg enzyme protein per kg dry matter.

### Claim 4 (depends on 1)

The process of claim 1, wherein the dosage of the laccase is 0.1-10 mg enzyme protein per kg dry matter.

### Claim 5 (depends on 1)

The process of claim 1, wherein the dosage of the laccase is 1-5 mg enzyme protein per kg dry matter.

### Claim 6 (depends on 1)

The process of claim 1, further comprising treating the zearalenone with a laccase mediator.

### Claim 7 (depends on 6)

The process of claim 6, wherein the laccase mediator is methylsyringate or phenothiazine-10-propionic acid.

### Claim 8 (depends on 6)

The process of claim 6, wherein the laccase mediator is N-(4-cyanophenyl)acetohydroxamic acid (NCPA), acetosyringone, syringaldehyde, p-coumaric acid, 2,2′-Azinobis(3-ethylbenzthiazoline-6-sulfonate), 1-hydroxybenzotriazole, 2,4-pentanedione, or phenothiazine.

### Claim 9 (depends on 2)

The process of claim 2, further comprising treating the zearalenone with a laccase mediator.

### Claim 10 (depends on 9)

The process of claim 9, wherein the laccase mediator is methylsyringate or phenothiazine-10-propionic acid.

### Claim 11 (depends on 9)

The process of claim 9, wherein the laccase mediator is N-(4-cyanophenyl)acetohydroxamic acid (NCPA), acetosyringone, syringaldehyde, p-coumaric acid, 2,2′-Azinobis(3-ethylbenzthiazoline-6-sulfonate), 1-hydroxybenzotriazole, 2,4-pentanedione, or phenothiazine.

### Claim 12 (depends on 1)

The process of claim 1, wherein the laccase has at least 95% sequence identity to SEQ ID NO: 1.

### Claim 13 (depends on 1)

The process of claim 1, wherein the laccase has at least 95% sequence identity to SEQ ID NO: 2.

### Claim 14 (depends on 1)

The process of claim 1, wherein the laccase has at least 95% sequence identity to SEQ ID NO: 3.

### Claim 15 (depends on 1)

The process of claim 1, wherein the zearalenone is in a feed product.

### Claim 16 (depends on 15)

The process of claim 15, wherein the feed product is a grain based feed product.

### Claim 17 (depends on 15)

The process of claim 15, wherein the feed product comprises one or more selected from the group consisting of corn, wheat, barley, rye, rice, sorghum and millet.

### Claim 18 (depends on 15)

The process of claim 15, wherein the feed product is an animal feed composition.

### Claim 19 (depends on 15)

The process of claim 15, wherein the feed product is a by-product from a fermentation process.

### Claim 20 (depends on 15)

The process of claim 15, wherein the feed product comprises brewers spent grain, distiller's spent grain, distiller's wet grain, and/or distiller's dried grain.