# US 9,040,274 B2 · Method for increasing protein thermal stability

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- Number: US 9,040,274 B2
- Document ID: US9040274B2
- Status: Granted
- Kind: B2
- Application number: 13/870,509
- Filed: 2013-04-25
- Published: 2014-10-30
- Granted: 2015-05-26
- Earliest claimed: 2012-05-10
- Assignees: Jiangnan University
- Applicants: Jian Chen; Guocheng Du; Xinyao Lu; Song Liu; Juan Zhang
- Inventors: Jian Chen; Guocheng Du; Xinyao Lu; Song Liu; Juan Zhang
- Web page: https://patentgrep.com/patent/US9040274B2

## CPC

- C12N 9/96: Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
- C12N 9/0069: {acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)}
- C12N 9/2411: {Amylases}
- C12N 9/1044: {Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The invention provides a simple and effective method for increasing thermal stability of a wide range of proteins, comprising fusing a self-assembling amphipathic peptide to the C- or N-terminal of target proteins. The fusion protein can have a half life up to 26 times longer than that of the wild type protein.

## Claims

### Claim 1

A method for increasing thermal stability of a target protein, comprising fusing a self-assembling amphipathic peptide (SAP) having an amino acid sequence set forth in SEQ ID NO:5 to said target protein by recombinant techniques to make a Target Protein-SAP fusion protein, wherein said Target protein-SAP fusion protein has increased thermal stability.

### Claim 2 (depends on 1)

The method of claim 1, wherein said SAP is fused to N-terminal or C-terminal of said target protein.