# US 9,040,273 B2 · Adsorption method for immobilizing the biomolecule and the biocatalysis system using the adsorption method thereof

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- Number: US 9,040,273 B2
- Document ID: US9040273B2
- Status: Granted
- Kind: B2
- Application number: 14/140,298
- Filed: 2013-12-24
- Published: 2014-11-20
- Granted: 2015-05-26
- Earliest claimed: 2013-05-16
- Assignees: Chung Yuan Christian University
- Applicants: Chung Yuan Christian University
- Inventors: Hsi-Ya Huang; Wan-Ling Liu; Chia-Her Lin; Sheng-Han Lo; Tadena Brenda Singco; Chun-Chuen Yang
- Web page: https://patentgrep.com/patent/US9040273B2

## CPC

- C12N 11/06: attached to the carrier via a bridging agent
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A simple, highly efficient, and environmentally friendly method of immobilizing macromolecules, particularly the biomolecules, to a solid carrier and thus forming a bioreactor, including the following steps: (1) providing the biomolecule and a leading molecule, and linking the biomolecule and the leading molecule by microwave to form an adsorbent; (2) providing a porous support material, and mixing the porous support material and the adsorbent to form a mixture; (3) treating the mixture by a vortex process such that the adsorbent being adsorbed and immobilized on the porous support material to form a bioreactor for biological reactions and biocatalysis; and the bioreactor can be further used for constructing a biological reaction system via incorporating a buffer solution and a ligand.

## Claims

### Claim 1

A molecule immobilization method of immobilizing a biomolecule to perform a biocatalysis, comprising the following steps of: providing the biomolecule and a small molecule which leads the biomolecule, and linking the biomolecule and the small molecule by microwave to form an adsorbent;
providing a porous support material, and mixing the porous support material and the adsorbent to form a mixture; and
treating the mixture by a vortex process such that the adsorbent being diffused and immobilized on the porous support material to form a bioreactor.

### Claim 2 (depends on 1)

The molecule immobilizing method of immobilizing a biomolecule to perform a biocatalysis according to claim 1, wherein the biomolecule is a protein enzyme; the small molecule is a dye; and the porous support material is a metal organic framework.

### Claim 3 (depends on 1)

The molecule immobilizing method of immobilizing a biomolecule to perform a biocatalysis according to claim 1, wherein a first duration of linking the biomolecule and the small molecule by microwave ranges from 1 minute to 5 minutes.

### Claim 4 (depends on 1)

The molecule immobilizing method of immobilizing a biomolecule to perform a biocatalysis according to claim 1, wherein a second duration of the vortex process ranges from 10 minutes to 60 minutes.

### Claim 5 (depends on 2)

The molecule immobilizing method of immobilizing a biomolecule to perform a biocatalysis according to claim 2, wherein the metal organic framework is an aluminum mental-organic framework material, and the aluminum mental-organic framework materials has a pore size ranging from 0.8 nm to 2.1 nm.

### Claim 6 (depends on 2)

The molecule immobilizing method of immobilizing a biomolecule to perform a biocatalysis according to claim 2, wherein the dye is fluorescein isothiocyanate.

### Claim 7 (depends on 2)

The molecule immobilizing method of immobilizing a biomolecule to perform a biocatalysis according to claim 2, wherein the protein enzyme is trypsin.