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AssigneeEVOLA Limited
InventorsMichael Katz, Hans Peter Smits, Jochen Förster, Jens Bredal Nielsen
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A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.
A genetically engineered micro-organism comprising an operative metabolic pathway that produces at least 0.25 milligrams resveratrol per gram on a dry weight basis of the genetically engineered micro-organism cells, said resveratrol having been produced in the cells, wherein the genetically engineered micro-organism has been recombinantly engineered to produce more than a native amount of a cytochrome P450 reductase (CPR), wherein said more than native amount of said CPR has been provided by replacing a native promoter of a gene expressing said CPR with a promoter providing a higher level of expression.
An isolated or non-naturally occurring genetically engineered mico-organism composition comprising (1) an isolated or normaturally occurring micro-organism cell having an operative metabolic pathway comprising at least one enzyme activity, said pathway producing 4-coumaric acid and producing resveratrol therefrom, and (2) resveratrol produced by said cell.
Dependencies are inferred from the claim text.