# US 9,040,257 B2 · Basidiomycetous yeast mutant

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- Number: US 9,040,257 B2
- Document ID: US9040257B2
- Status: Granted
- Kind: B2
- Application number: 14/394,691
- Filed: 2013-04-23
- Published: 2015-03-12
- Granted: 2015-05-26
- Earliest claimed: 2012-05-17
- Assignees: Toyobo Co., Ltd.; National Research Institute of Brewing
- Applicants: Toyobo Co., Ltd.; National Research Institute of Brewing
- Inventors: Yuu Utashima; Takahide Kishimoto; Shusaku Yanagidani; Kazuo Masaki
- Web page: https://patentgrep.com/patent/US9040257B2

## CPC

- C12N 1/16: Yeasts; Culture media therefor
- C12N 1/145: Fungi isolates
- C12P 21/00: Preparation of peptides or proteins (single cell protein C12N1/00)
- C12P 21/02: having a known sequence of two or more amino acids, e.g. glutathione
- C12R 2001/645: Fungi {; Processes using fungi}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present invention provides a host for genetic recombination useful in the production of heterologous proteins in a large scale, by suppressing the production of extracellular polysaccharides in the basidiomycetous yeasts. Cryptococcus sp. S-2 D11 strain (FERM BP-11482) which is characterized in that the production of extracellular polysaccharides is suppressed as compared with the parent strain.

## Claims

### Claim 1

A Cryptococcus strain deposited as FERM BP-11482.

### Claim 2

A method for producing a target protein, comprising:
transforming a gene of the target protein to a Cryptococcus strain deposited as FERM BP-11482,
culturing the resulting transformant, and
collecting the target protein from the resulting culture product.