# US 9,040,251 B2 · Biomolecule fixing board and method of manufacturing the same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,040,251 B2 - Document ID: US9040251B2 - Status: Granted - Kind: B2 - Application number: 13/388,671 - Filed: 2010-07-29 - Published: 2012-05-24 - Granted: 2015-05-26 - Earliest claimed: 2009-08-04 - Assignees: Hitachi High-Technologies Corporation - Applicants: Yasuhiko Tada; Hiroshi Yoshida; Toshiro Saito; Masatoshi Narahara; Hiroaki Nakagawa - Inventors: Yasuhiko Tada; Hiroshi Yoshida; Toshiro Saito; Masatoshi Narahara; Hiroaki Nakagawa - Web page: https://patentgrep.com/patent/US9040251B2 ## CPC - G01N 33/54353: {with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393)} - G01N 33/54393: {Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding} - A61K 2039/625: {binding through the biotin-streptavidin system or similar} - G01N 33/543: with an insoluble carrier for immobilising immunochemicals - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract This invention provides a biomolecule modifying substrate comprising biomolecules selectively fixed to given regions thereon. The biomolecule modifying substrate comprises: a substrate at least comprising a first surface and a second surface; a first linker molecule comprising a hydrocarbon chain and a functional group capable of selectively binding to the first surface at one end of the hydrocarbon chain, which is bound to the first surface via such functional group; a second linker molecule comprising a reactive group capable of binding to the hydrocarbon chain of the first linker molecule, which is bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; and a biomolecule bound thereto via the second linker molecule. ## Claims ### Claim 1 A biomolecule modifying substrate comprising: a substrate; a first surface on the substrate, the first surface comprising a metal oxide; a second surface on the substrate, the second surface comprising a noble metal; a first linker molecule comprising a hydrocarbon chain and a functional group at one end or in a side chain of the hydrocarbon chain, the functional group capable of selectively binding to the first surface, the first linker molecule bound to the first surface via the functional group; a second linker molecule comprising biotin and a reactive group, the reactive group capable of binding to the hydrocarbon chain of the first linker molecule, the second linker molecule bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; a biomolecule comprising an introduced biotin; and an avidin, wherein the biotin contained in the second linker molecule is bound to the biotin introduced into the biomolecule via the avidin, and wherein the functional group of the first linker molecule capable of selectively binding to the first surface is a phosphate group. ### Claim 2 (depends on 1) The biomolecule modifying substrate according to claim 1, wherein the first surface is a metal having a natural oxide film. ### Claim 3 (depends on 1) The biomolecule modifying substrate according to claim 1, wherein the hydrocarbon chain of the first linker molecule comprises an alkyl group and is represented by the formula selected from the group consisting of CmH2m-1PO3H2, CmH2m-1OPO3H2, wherein m is an integer between 2 and 20. ### Claim 4 (depends on 1) The biomolecule modifying substrate according to claim 1, wherein the hydrocarbon chain of the first linker molecule is a polymer chain comprising a phosphate group in the side chain of a compound represented by formula 3: wherein n is an integer of 1 or larger. ### Claim 5 (depends on 1) The biomolecule modifying substrate according to claim 1, wherein the reactive group in the second linker molecule is a latent reactive group capable of binding to the hydrocarbon chain and the latent reactive group is at least 1 type of photoreactive compound selected from the group consisting of an anthrathione group, an anthraquinone group, a benzophenone group, an azide group, and a derivative of any thereof. ### Claim 6 (depends on 1) The biomolecule modifying substrate according to claim 1, wherein the second linker molecule further comprises a reactive group capable of selectively binding to the biomolecule or a reactive group bound to the biomolecule. ### Claim 7 (depends on 1) The biomolecule modifying substrate according to claim 1, wherein the first linker molecule lacks a functional group inhibiting binding between the second linker molecule and the hydrocarbon chain of the first linker molecule, and wherein the first linker molecule lacks a functional group capable of binding to the second surface. ### Claim 8 (depends on 1) The biomolecule modifying substrate according to claim 1, wherein the hydrocarbon chain of the first linker molecule comprises an alkyl group, and wherein functional group of the second linker molecule is bonded to the alkyl group of the first linker molecule.