# US 9,040,247 B2 · Detection of human T1R expressing cells

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- Number: US 9,040,247 B2
- Document ID: US9040247B2
- Status: Granted
- Kind: B2
- Application number: 13/600,377
- Filed: 2012-08-31
- Published: 2013-03-14
- Granted: 2015-05-26
- Earliest claimed: 2000-03-07
- Assignees: Senomyx, Inc.
- Applicants: Jon Elliot Adler; Sergey Zozulya; Xiaodong Li; Shawn O'Connell; Lena Staszewski
- Inventors: Jon Elliot Adler; Sergey Zozulya; Xiaodong Li; Shawn O'Connell; Lena Staszewski
- Web page: https://patentgrep.com/patent/US9040247B2

## CPC

- C07K 14/723: {G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor}
- C07K 14/705: Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)}
- C07K 2319/00: Fusion polypeptide
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.

## Claims

### Claim 1

A method of identifying T1R1 expressing cells comprising detecting cells that express a T1R1 taste receptor, wherein such taste receptor comprises a taste receptor polypeptide that is at least 90% identical to the T1R1 polypeptide of SEQ ID NO:17.

### Claim 2 (depends on 1)

The method of claim 1 which uses an antibody that specifically detects the expression of said polypeptide.

### Claim 3 (depends on 1)

The method of claim 1, wherein said step of detecting cells uses a probe comprising an antibody or antibody fragment having binding specificity to said taste receptor polypeptide.

### Claim 4 (depends on 1)

The method of claim 1 wherein said detected T1R1 expressing cell is isolated.

### Claim 5 (depends on 1)

The method of claim 1 wherein said T1R1 expressing cell endogenously expresses said taste receptor polypeptide.

### Claim 6 (depends on 1)

The method of claim 1 wherein said T1R1 expressing cell further expresses a T1R3 polypeptide.

### Claim 7 (depends on 5)

The method of claim 5 wherein said T1R1 expressing cell further expresses a T1R3 polypeptide.

### Claim 8 (depends on 6)

The method of claim 6 wherein said T1R1 expressing cell endogenously expresses said T1R3 polypeptide.

### Claim 9 (depends on 7)

The method of claim 7 wherein said T1R1 expressing cell endogenously expresses said T1R3 polypeptide.

### Claim 10 (depends on 1)

The method of claim 1, wherein said T1R1 polypeptide has the sequence of SEQ ID NO:17.