# US 9,040,245 B2 · Methods, devices, kits and compositions for detecting roundworm, whipworm, and hookworm > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,040,245 B2 - Document ID: US9040245B2 - Status: Granted - Kind: B2 - Application number: 13/110,487 - Filed: 2011-05-18 - Published: 2013-06-13 - Granted: 2015-05-26 - Earliest claimed: 2007-06-15 - Assignees: IDEXX Laboratories, Inc.; Divergence, Inc. - Applicants: David Allen Elsemore; Jinming Geng; Laurie A. Flynn; Michael Crawford - Inventors: David Allen Elsemore; Jinming Geng; Laurie A. Flynn; Michael Crawford - Web page: https://patentgrep.com/patent/US9040245B2 ## CPC - G01N 33/56966: {Animal cells} - G01N 33/5308: {for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites} - G01N 33/6893: {related to diseases not provided for elsewhere} - G01N 2333/43526: from worms - G01N 2333/4353: from nematodes - G01N 2469/10: Detection of antigens from microorganism in sample from host - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Methods, devices, kits and compositions for detecting the presence or absence of one or more helminthic coproantigens in a sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm, whipworm and/or hookworm in a fecal sample from a mammal and may also be able to distinguish between one or more helminth infections. Confirmation of the presence or absence of roundworm, whipworm and/or hookworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated. ## Claims ### Claim 1 A method of detecting the presence or absence of one or more helminthic antigens in a sample, the method comprising contacting a sample from a mammal with: (a) a first antibody capable of specifically binding a roundworm coproantigen, but not a whipworm coproantigen; (b) a second antibody capable of specifically binding a whipworm coproantigen, but not a roundworm coproantigen; (c) forming antibody-coproantigen complexes in the presence of the coproantigens, if any, in the sample; and (d) detecting the presence or absence of the antibody-coproantigen complexes, if any. ### Claim 2 A method of detecting the presence or absence of one or more helminthic antigens in a sample, the method comprising contacting a sample from a mammal with: (a) a first antibody capable of specifically binding a roundworm coproantigen, but not a hookworm coproantigen; (b) a second antibody capable of specifically binding a hookworm coproantigen, but not a roundworm coproantigen; (c) forming antibody-coproantigen complexes in the presence of the coproantigens, if any, in the sample; and (d) detecting the presence or absence of the antibody-coproantigen complexes, if any. ### Claim 3 A method of detecting the presence or absence of one or more helminthic antigens in a sample, the method comprising contacting a sample from a mammal with: (a) a first antibody capable of specifically binding a whipworm coproantigen, but not a hookworm coproantigen; (b) a second antibody capable of specifically binding a hookworm coproantigen, but not a whipworm coproantigen; (c) forming antibody-coproantigen complexes in the presence of the coproantigens, if any, in the sample; and (d) detecting the presence or absence of the antibody-coproantigen complexes, if any. ### Claim 4 (depends on 1, 2) The method of any one of claim 1, 2, or 3, further comprising the step of contacting the sample with one or more reagents to detect one or more of the group consisting of: one or more non-worm parasites, heartworm, one or more viruses, one or more fungi, and one or more bacteria. ### Claim 5 (depends on 4) The method of claim 4, wherein the reagents for the detection of any one or all of the one or more non-worm parasites, one or more viruses, one or more fungi and the one or more bacteria are one or more antibodies or one or more antigens recognized by antibodies specific for one or more non-worm parasites, one or more viruses, one or more fungi or one or more bacteria. ### Claim 6 (depends on 1, 2) The method of any one of claim 1 or 2, wherein the antibody capable of specifically binding a roundworm coproantigen is capable of specifically binding a polypeptide consisting of an amino acid sequence of SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 38, or Copro6728; or the antibody was raised against an extract of whole roundworms, or extract of roundworm reproductive organs, or extract of roundworm intestines. ### Claim 7 (depends on 1, 3) The method of any one of claim 1 or 3, wherein the antibody capable of specifically binding a whipworm coproantigen is capable of specifically binding a polypeptide consisting of an amino acid sequence of SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 9. ### Claim 8 (depends on 2, 3) The method of any one of claim 2 or 3, wherein the antibody capable of specifically binding a hookworm coproantigen is capable of specifically binding a polypeptide consisting of an amino acid sequence of SEQ ID NO:33 or SEQ ID NO: 34, or CoproASP5. ### Claim 9 (depends on 1, 2) The method of any one of claim 1, 2, or 3, wherein the sample is obtained from a mammal that is a canine or a feline. ### Claim 10 (depends on 1, 2) The method of any one of claim 1 or 2, wherein the roundworm is Toxocara canis or Toxocara cati, Toxocara vitulorum, Toxascaris leonina, Baylisascaris procyonis, Ascaridia galli, Parascaris equorum, Ascaris suum, Ascaris lumbricoides, Anisakis simplex, or Pseudoterranova decipiens. ### Claim 11 (depends on 1, 3) The method of any one of claim 1 or 3, wherein the whipworm is Trichuris vulpis, Trichuris campanula, Trichuris serrata, Trichuris suis, Trichuris trichiura, Trichuris discolor and Trichocephalus trichiuris. ### Claim 12 (depends on 2, 3) The method of any one of claim 2 or 3, wherein the hookworm is Ancylostoma caninum, Ancylostoma braziliense, Ancylostoma duodenal, Ancylostoma ceylanicum, Ancylostoma tubaeforme and Ancylostoma pluridentatum, Necator americanus, and Uncinaria stenocephala. ### Claim 13 (depends on 1, 2) The method of any one of claim 1, 2, or 3, wherein the first and second antibodies do not specifically bind any coproantigen derived from heartworm. ### Claim 14 (depends on 1, 2) The method of any one of claim 1, 2, or 3, wherein the step of detecting the presence or absence of the complexes further includes the step of providing at least one secondary antibody that binds to at least one of the complexes. ### Claim 15 (depends on 14) The method of claim 14, wherein the at least one secondary antibody is labeled. ### Claim 16 (depends on 1, 2) The method of any one of claim 1, 2, or 3, wherein one or more of the first and second antibodies are labeled. ### Claim 17 (depends on 1, 2) The method of any one of claim 1, 2, or 3, wherein the first and second antibodies are immobilized on a solid support. ### Claim 18 (depends on 17) The method of claim 17, wherein the solid support forms part of an enzyme-linked immunosorbent assay device. ### Claim 19 (depends on 18) The method of claim 18, wherein the enzyme-linked immunosorbent assay device is a lateral flow immunoassay device. ### Claim 20 (depends on 1, 2) The method of any one of claim 1, 2, or 3, wherein the roundworm coproantigen, hookworm coproantigen, and/or whipworm coproantigen is from a fecal sample. ### Claim 21 A method of diagnosing whether a mammal is infected with one or more parasitic worms, the method comprising the steps of: (a) contacting a sample from a mammal with: (i) a first antibody capable of specifically binding a roundworm coproantigen, but not a whipworm coproantigen; and (ii) a second antibody capable of specifically binding a whipworm coproantigen, but not a roundworm coproantigen; (b) forming antibody-coproantigen complexes in the presence of the coproantigens, if any, in the sample; (c) detecting the presence or absence of the antibody-coproantigen complexes, if any; and (d) diagnosing the mammal as having: (i) a roundworm infection if a roundworm antibody-coproantigen complex is present; and (ii) a whipworm infection if a whipworm antibody-coproantigen complex is present. ### Claim 22 A method of diagnosing whether a mammal is infected with one or more parasitic worms, the method comprising the steps of: (a) contacting a sample from a mammal with: (i) a first antibody capable of specifically binding a roundworm coproantigen, but not a hookworm coproantigen; (ii) a second antibody capable of specifically binding a hookworm coproantigen, but not a roundworm coproantigen; (b) forming antibody-coproantigen complexes in the presence of the coproantigens, if any, in the sample; (c) detecting the presence or absence of the antibody-coproantigen complexes, if any; and (d) diagnosing the mammal as having: (i) a roundworm infection if a roundworm antibody-coproantigen complex is present; and (ii) a hookworm infection if a hookworm antibody-coproantigen complex is present. ### Claim 23 A method of diagnosing whether a mammal is infected with one or more parasitic worms, the method comprising the steps of: (a) contacting a sample from a mammal with: (i) a first antibody capable of specifically binding a whipworm coproantigen, but not a hookworm coproantigen; (ii) a second antibody capable of specifically binding a hookworm coproantigen, but not a whipworm coproantigen; (b) forming antibody-coproantigen complexes in the presence of the coproantigens, if any, in the sample; (c) detecting the presence or absence of the antibody-coproantigen complexes, if any; and (d) diagnosing the mammal as having: (i) a whipworm infection if a whipworm antibody-coproantigen complex is present; and (ii) a hookworm infection if a hookworm antibody-coproantigen complex is present.