# US 9,040,057 B2 · Method of diagnosing and preventing pneumococcal diseases using pneumococcal neuraminidases

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- Number: US 9,040,057 B2
- Document ID: US9040057B2
- Status: Granted
- Kind: B2
- Application number: 13/244,482
- Filed: 2011-09-25
- Published: 2013-03-28
- Granted: 2015-05-26
- Earliest claimed: 2011-09-25
- Assignees: Chang Gung Medical Foundation, Linkou Branch
- Applicants: Cheng-Hsun Chiu; Rajendra Prasad Janapatla; Chyi-Liang Chen; Mei-Hua Hsu; Hsiu-Ling Chen; Chung-Tsui Huang; Hsin-Ju Chang; Wan-Ting Liao
- Inventors: Cheng-Hsun Chiu; Rajendra Prasad Janapatla; Chyi-Liang Chen; Mei-Hua Hsu; Hsiu-Ling Chen; Chung-Tsui Huang; Hsin-Ju Chang; Wan-Ting Liao
- Web page: https://patentgrep.com/patent/US9040057B2

## CPC

- A61K 39/092: {Streptococcus}
- A61P 31/04: Antibacterial agents
- A61P 37/04: Immunostimulants
- G01N 33/573: for enzymes or isoenzymes
- A61K 2039/505: {comprising antibodies}
- G01N 2333/924: acting on glycosyl compounds (3.2)
- G01N 2500/02: Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A method of providing protection against pneumococcal infection in a subject is disclosed. The method includes steps of administering to the subject a composition that includes combination of three recombinant pneumococcal neuraminidases: NanA, NanB, and NanC of S. pneumoniae strains CGSP14, wherein administration of the recombinant pneumococcal neuraminidases elicits an immune response to S. pneumoniae, and treats the subject. In one embodiment, the method further includes a step of adding adjuvants to enhance the immune response. The method also includes a step of using passive antibodies, wherein said passive antibodies are anti-neuraminidase antibodies generated from neuraminidases-immunized humanized animals: NanA, NanB, and NanC. Meanwhile, this invention also provides a method for the molecular diagnosis of pneumococcal infection.

## Claims

### Claim 1

A method of providing protection against pneumococcal infection in a subject comprises steps of:
administering immunogenic amount of composition that includes the combination of three recombinant pneumococcal neuraminidases: NanA, NanB, and NanC of Streptococcus pneumoniae to the subject;
wherein the recombinant pneumococcal neuraminidase NanA is SEQ ID NO: 4;
wherein the recombinant pneumococcal neuraminidase NanB is SEQ ID NO: 5;
wherein the recombinant pneumococcal neuraminidase NanC is SEQ ID NO: 6;
wherein administering the recombinant pneumococcal neuraminidases elicits an immune response to Streptococcus pneumoniae.

### Claim 2 (depends on 1)

The method of providing protection against pneumococcal infection in a subject of claim 1, further comprises a step of adding adjuvants to enhance the immune response.

### Claim 3 (depends on 1)

The method of providing protection against pneumococcal infection in a subject of claim 1, wherein said Streptococcus pneumoniae includes all kinds of Streptococcus pneumoniae serotypes.

### Claim 4 (depends on 1)

The method of providing protection against pneumococcal infection in a subject of claim 1, wherein the subject is human.

### Claim 5 (depends on 1)

The method of providing protection against pneumococcal infection in a subject of claim 1, wherein the subject is animal.