# US 9,039,896 B2 · Device for removing protein-based substances > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,039,896 B2 - Document ID: US9039896B2 - Status: Granted - Kind: B2 - Application number: 13/911,516 - Filed: 2013-06-06 - Published: 2013-10-17 - Granted: 2015-05-26 - Earliest claimed: 2003-05-12 - Assignees: Hepa Wash GmbH - Applicants: Bernhard Kreymann - Inventors: Bernhard Kreymann - Web page: https://patentgrep.com/patent/US9039896B2 ## CPC - A61M 1/16: with membranes - A61M 1/1696: {with dialysate regeneration} - A61M 1/3472: {with treatment of the filtrate} - A61M 1/3482: {by filtrating the filtrate using another cross-flow filter, e.g. a membrane filter} - A61M 1/3486: {Biological, chemical treatment, e.g. chemical precipitation; treatment by absorbents} - A61M 1/3493: {using treatment agents in suspension} - A61M 1/3623: {Means for actively controlling temperature of blood} - A61M 1/369: {Temperature treatment} - A61M 2205/058: with ultrasound therapy - A61M 2205/3368: Temperature - A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)} ## Abstract The present invention relates to a means of dialysis for removing protein-bound substances from a biological fluid, especially blood or blood plasma, which contains at least one means for solubilizing protein-binding substances to be removed into the biological fluid and/or dialysis fluid, and to a process for removing protein-bound substances from a biological fluid. ## Claims ### Claim 1 A method for dialysis of a biological fluid containing at least one protein-binding substance to be removed, comprising the following steps: (a) passing the biological fluid through a first chamber of a first dialyser wherein the first chamber forms part of a biological fluid circuit, (b) passing a dialysis fluid containing a substance that functions as an adsorber for the substance to be removed from the biological fluid through a second chamber of the first dialyser wherein the second chamber forms part of a dialysis fluid circuit, (c) at least partially solubilizing the protein binding substance to be removed in the dialysis fluid by adjusting the pH of the dialysis fluid to 1-7 upstream of a second dialyser, (d) passing the dialysis fluid with the pH adjusted to 1-7 through the second dialyser forming part of the dialysis fluid circuit, and (e) adjusting the pH of the dialysis fluid to 8-13 downstream of the second dialyser. ### Claim 2 (depends on 1) The process according to claim 1 wherein dialysable compounds binding to the substances to be removed are added to the dialysis fluid and/or biological fluid. ### Claim 3 (depends on 2) The process according to claim 2, wherein said compounds include caffeine and chelating agents for metal cations. ### Claim 4 (depends on 3) The process according to claim 3, wherein penicillamine, trientine, deferoxamine, preferiprone, HBED, vitamin C, BAL, DMPS or DMSA is the chelating agent. ### Claim 5 (depends on 1) The method according to claim 1, wherein the pH of the dialysis fluid is adjusted by adding acid. ### Claim 6 (depends on 1) The method according to claim 1 further comprising heating or cooling the dialysis fluid. ### Claim 7 (depends on 6) The method according to claim 6, wherein heating is performed by a heating apparatus, a microwave apparatus or an infrared apparatus. ### Claim 8 (depends on 6) The method according to claim 6 wherein the cooling is performed by a cooling unit. ### Claim 9 (depends on 1) The method according to claim 1 further comprising passing the dialysis fluid through at least one additional dialyser in the dialysis fluid circuit. ### Claim 10 (depends on 6) The method according to claim 6 wherein the dialysis fluid is heated to 41-45° C. ### Claim 11 (depends on 1) The method according to claim 1 wherein the adsorber comprises albumin. ### Claim 12 (depends on 1) The method according to claim 1, wherein the dialysis fluid contains human serum albumin in a concentration of 1 to 25 g per 100 ml. ### Claim 13 (depends on 1) The method according to claim 1 further comprising adding a substituate to dilute or change the salt content of the dialysis fluid. ### Claim 14 (depends on 1) The method according to claim 1 further comprising recirculating the dialysis fluid. ### Claim 15 (depends on 1) The method according to claim 1 wherein the second dialyser is a filter. ### Claim 16 (depends on 9) The method according to claim 9 wherein the pH of the dialysis fluid is adjusted to 8-13 upstream of the at least one additional dialyser in the dialysis fluid circuit. ### Claim 17 (depends on 16) The method according to claim 16 wherein the pH of the dialysis fluid is adjusted to 8-13 by adding base. ### Claim 18 (depends on 1) The method according to claim 1 further comprising providing a means for adjusting the pH of the dialysis fluid to substantially neutral. ### Claim 19 (depends on 1) The method according to claim 1 wherein (c) the protein binding substance to be removed in the dialysis fluid is at least partially solubilized by adjusting the pH of the dialysis fluid to 2.5-5 upstream of a second dialyser.