# US 9,039,880 B2 · Device and method for manufacturing the same

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- Number: US 9,039,880 B2
- Document ID: US9039880B2
- Status: Granted
- Kind: B2
- Application number: 13/242,761
- Filed: 2011-09-23
- Published: 2012-06-07
- Granted: 2015-05-26
- Earliest claimed: 2010-12-01
- Assignees: ARKRAY, Inc.
- Applicants: Koji Sugiyama; Rina Matsumi; Satoshi Yonehara
- Inventors: Koji Sugiyama; Rina Matsumi; Satoshi Yonehara
- Web page: https://patentgrep.com/patent/US9039880B2

## CPC

- G01N 27/44791: {Microapparatus (sample containers with integrated microfluidic structures B01L3/5027)}
- B01L 3/502707: {characterised by the manufacture of the container or its components}
- B32B 3/02: characterised by features of form at particular places, e.g. in edge regions {(non-uniform thickness B32B3/263)}
- B32B 3/266: {characterised by an apertured layer, the apertures going through the whole thickness of the layer, e.g. expanded metal, perforated layer, slit layer regular cells B32B3/12}
- B32B 3/30: characterised by a layer formed with recesses or projections, e.g. {hollows, grooves, protuberances, ribs (apertured layer B32B3/266; layer with cavities or internal voids B32B3/26)}
- B32B 7/10: at least one layer having inter-reactive properties
- B32B 7/12: using interposed adhesives or interposed materials with bonding properties
- B32B 27/08: of synthetic resin
- B32B 27/16: specially treated, e.g. irradiated
- B32B 38/00: Ancillary operations in connection with laminating processes
- C09J 5/02: involving pretreatment of the surfaces to be joined
- B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions
- B01L 2400/0421: electrophoretic flow
- B29C 66/026: {Chemical pre-treatments (B29C66/028 takes precedence)}
- B29C 66/028: {Non-mechanical surface pre-treatments, i.e. by flame treatment, electric discharge treatment, plasma treatment, wave energy or particle radiation (B29C65/14 takes precedence; non-mechanical surface treatment of plastics in general B29C59/08 - B29C59/16)}
- B29C 66/53461: {joining substantially flat covers and/or substantially flat bottoms to open ends of container bodies}
- B29C 66/54: {Joining several hollow-preforms, e.g. half-shells, to form hollow articles, e.g. for making balls, containers; Joining several hollow-preforms, e.g. half-cylinders, to form tubular articles}
- B29L 2031/756: {Microarticles, nanoarticles}
- B32B 27/308: {comprising acrylic (co)polymers}
- B32B 37/0076: {characterised in that the layers are not bonded on the totality of their surfaces}
- B32B 37/203: {One or more of the layers being plastic}
- B32B 38/0004: {Cutting, tearing or severing, e.g. bursting; Cutter details (cutting in general B26D; laminating combined with punching or perforating B32B38/04; removing all or part of the layers B32B38/10; cutting in combination with laying up and registration B32B38/185 takes precedence)}
- B32B 2037/0092: {in which absence of adhesives is explicitly presented as an advantage}
- B32B 2038/0076: {Curing, vulcanising, cross-linking}
- B32B 2250/02: 2 layers
- B32B 2250/24: All layers being polymeric
- B32B 2333/12: Polymers of methacrylic acid esters, e.g. PMMA, i.e. polymethylmethacrylate
- Y10T 428/24562: Interlaminar spaces
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B32B: LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- C09J: ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES (preparation of glue or gelatine C09H)
- B29C: SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING (making preforms B29B11/00; making laminated products by combining previously unconnected layers which become one product whose layers will remain together B32B37/00 - B32B41/00)
- B29L: INDEXING SCHEME ASSOCIATED WITH SUBCLASS B29C, RELATING TO PARTICULAR ARTICLES
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

The present invention provides a device that decreases deformation during manufacturing of the device, provides a firm joint without use of an adhesive, and allows chemical modification of a channel during manufacturing of the device. The device includes two joined substrates, and a concavity is formed on at least one of the opposing surfaces of the two substrates so as to make a channel, where the two substrates are joined together by a covalent bond via a crosslinking agent (A), and the crosslinking agent (A) is exposed on an inner wall surface of the channel.

## Claims

### Claim 1

A device comprising two joined substrates, where a concavity is formed on at least one of opposing surfaces of the two substrates so as to make a channel,
wherein the two substrates are joined together by a covalent bond via a crosslinking agent (A), and
the crosslinking agent (A) is exposed on an inner wall surface of the channel, the crosslinking agent (A) consisting of an ionic group containing polysaccharide.

### Claim 2 (depends on 1)

The device according to claim 1, wherein the channel is a capillary, an aqueous solution is arranged and a voltage is applied in the channel and an electroosmotic flow is generated.

### Claim 3 (depends on 1)

The device according to claim 1, wherein the crosslinking agent (A) is bonded to a functional group (C) on the substrate surface via a crosslinking agent (B), the crosslinking agent (A) and the crosslinking agent (B) are covalently bonded to each other, and the crosslinking agent (B) and the functional group (C) are covalently bonded to each other.

### Claim 4 (depends on 1)

The device according to claim 1, wherein the crosslinking agent (A) is bonded to a functional group (C) on the substrate surface, and the crosslinking agent (A) and the functional group (C) are covalently bonded to each other.

### Claim 5 (depends on 1)

The device according to claim 1, wherein the covalent bond is selected from the group consisting of a Schiff's base formation between an amino group and an aldehyde group, an amide bond between an amino group and a carboxyl group, a copolymerization between double bonds, and an ether bond between a hydroxyl group and an epoxy group.

### Claim 6 (depends on 3)

The device according to claim 3, wherein the crosslinking agent (B) is a dialdehyde having aldehyde groups at both terminals.

### Claim 7 (depends on 3)

The device according to claim 3, wherein the functional group (C) is selected from the group consisting of an amino group, a carboxyl group, a methoxy group, a vinyl group, an aldehyde group, and a hydroxyl group.

### Claim 8

A method for manufacturing a device that comprises two joined substrates, wherein a concavity is formed on at least one of opposing surfaces of the two substrates so as to make a channel, the method comprising:
bringing a crosslinking agent (A) into contact with the opposing surfaces of the two substrates, superimposing the two substrates and causing a reaction between the superimposed two substrates, so that the two substrates join together by a covalent bond via the crosslinking agent (A) and that the crosslinking agent (A) is exposed on an inner wall surface of the channel, the crosslinking agent (A) consisting of an ionic group containing polysaccharide.

### Claim 9 (depends on 8)

The method for manufacturing a device according to claim 8, further comprising:
bringing a crosslinking agent (B) into contact and reaction with a functional group (C) present on the opposing surfaces of the two substrates so as to introduce the crosslinking agent (B) onto the two substrates by a covalent bond; and
applying the crosslinking agent (A) on one of the two substrates onto which the crosslinking agent (B) has been introduced, superimposing the other of the two substrates so as to cause a reaction between the crosslinking agent (A) and the crosslinking agent (B), and joining the two substrates together by the covalent bond while modifying the interior of the thus formed channel with the crosslinking agent (A).

### Claim 10 (depends on 8)

The method for manufacturing a device according to claim 8, further comprising:
bringing the crosslinking agent (A) and a crosslinking agent (B) into contact and reaction so as to make a crosslinking agent bound substance (A-B); and
applying the crosslinking agent bound substance (A-B) on one of the pair of substrates having the functional group (C) present on the surface, superimposing the other substrate so as to cause a reaction between the crosslinking agent (B) and the functional group (C), and joining the substrates together by the covalent bond while modifying the interior of the thus formed channel with the crosslinking agent (A).

### Claim 11 (depends on 8)

The method for manufacturing a device according to claim 8, further comprising:
applying the crosslinking agent (A) on one of the two substrates having a functional group (C) present on the surface, superimposing the other substrate so as to cause a reaction between the crosslinking agent (A) and the functional group (C), and joining the substrates together by the covalent bond while modifying the interior of the thus formed channel with the crosslinking agent (A).

### Claim 12 (depends on 10)

The method for manufacturing a device according to claim 10, further comprising introducing the functional group (C) onto the opposing substrate surface.

### Claim 13 (depends on 10)

The method for manufacturing a device according to claim 10, wherein the crosslinking agent (B) is a dialdehyde having aldehyde groups at both terminals.

### Claim 14 (depends on 9)

The method for manufacturing a device according to claim 9, wherein the functional group (C) is selected from the group consisting of an amino group, a carboxyl group, a methoxy group, a vinyl group, an aldehyde group, and a hydroxyl group.

### Claim 15 (depends on 1)

A method for measuring an analytical object present in a sample, comprising
introducing the sample to the device according to claim 1,
performing a separation analysis of the sample by capillary electrophoresis; and
measuring the amount of the separated analytical object.

### Claim 16 (depends on 1)

An analyzing system comprising the device according to claim 1 and a means for measuring an analytical object present in a sample, where the means for measuring comprises a means for mounting the device and for performing an analysis of the sample.