# US 9,038,689 B2 · Micro-fluidic partitioning between polymeric sheets for chemical amplification and processing

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- Number: US 9,038,689 B2
- Document ID: US9038689B2
- Status: Granted
- Kind: B2
- Application number: 13/480,274
- Filed: 2012-05-24
- Published: 2012-11-29
- Granted: 2015-05-26
- Earliest claimed: 2011-05-26
- Assignees: Lawrence Livermore National Security, LLC
- Applicants: Brian L. Anderson
- Inventors: Brian L. Anderson
- Web page: https://patentgrep.com/patent/US9038689B2

## CPC

- B01L 7/52: {with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples}
- B32B 37/0038: {involving application of liquid to the layers prior to lamination, e.g. wet laminating (B32B37/12 takes precedence)}
- B32B 37/1027: {Pressing using at least one press band}
- B32B 37/182: {one or more of the layers being plastic}
- B32B 38/0008: {Electrical discharge treatment, e.g. corona, plasma treatment; wave energy or particle radiation (heat treatment B32B38/0036)}
- C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence)
- B65B 9/023: {Packaging fluent material (B65B9/04 takes precedence)}
- Y10T 156/1741: Progressive continuous bonding press [e.g., roll couples]
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B32B: LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- B65B: MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING (bundling and pressing devices for cigars A24C1/44; devices for tensioning and securing binders adapted to be supported by the article or articles to be bound B25B25/00; applying closure members to bottles, jars or similar containers B67B1/00-B67B6/00; concurrent cleaning, filling and closing of bottles B67C7/00; emptying bottles, jars, cans, casks, barrels or similar containers B67C9/00)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

A system for fluid partitioning for chemical amplification or other chemical processing or separations of a sample, comprising a first dispenser of a first polymeric sheet, wherein the first polymeric sheet contains chambers; a second dispenser of a second polymeric sheet wherein the first dispenser and the second dispenser are positioned so that the first polymeric sheet and the second polymeric sheet become parallel; a dispenser of the fluid positioned to dispense the fluid between the first polymeric sheet and the second polymeric sheet; and a seal unit that seals the first polymeric sheet and the second polymeric sheet together thereby sealing the sample between the first polymeric sheet and the second polymeric sheet and partitioning the fluid for chemical amplification or other chemical processing or separations.

## Claims

### Claim 1

An apparatus for fluid partitioning a sample for polymerase chain reaction amplification of the sample, consisting of:
a first polymeric sheet in a first roll;
a first dispenser of said first polymeric sheet from said first roll wherein said first dispenser dispenses said first polymeric sheet linearly;
a second polymeric sheet in a second roll;
a second dispenser of said second polymeric sheet from said second roll wherein said second dispenser dispenses said second polymeric sheet linearly and wherein said first dispenser and said second dispenser are positioned so that said first polymeric sheet and said second polymeric sheet become parallel;
a space between said first polymeric sheet and said second polymeric sheet;
a sample dispenser positioned to dispense the sample into said space between said first polymeric sheet and said second polymeric sheet; polymerase chain reaction amplification reagents;
a polymerase chain reaction amplification reagents dispenser positioned to dispense said polymerase chain reaction amplification reagents into said space between said first polymeric sheet and said second polymeric sheet together with the sample;
a seal unit that seals said first polymeric sheet and said second polymeric sheet together thereby sealing the sample and said polymerase chain reaction amplification reagents in said space between said first polymeric sheet and said second polymeric sheet and partitioning the sample for polymerase chain reaction amplification of the sample, wherein said seal unit that seals said first polymeric sheet and said second polymeric sheet together comprises a first roller with longitudinal projections and a second roller with transverse projections; and
a roller with projections that create indentations in said first polymeric sheet.