Fluorescent polymeric materials containing lipid soluble rhodamine dyes
AssigneeApplied Biosystems, LLC
InventorsJoe Y. L. Lam, Steven Menchen, Ruiming Zou, Scott Benson
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Abstract
Fluorescent polymeric materials are disclosed comprising a polymer and one or more lipid soluble rhodamine dyes with the following core structure: The materials are especially useful in the preparation of multicolored microparticles, especially multicolored polystyrene microparticles, for use in the multiplexed analysis of a plurality of analytes in a single sample. When excited by a light source, the materials give off a unique emission based on the nature, concentration and ratio of the dyes therein. Methods of preparing and using the fluorescent polymeric materials are also disclosed.
Claims12
A fluorescent polymeric material comprising a polymer and at least one lipid-soluble rhodamine dye comprising the following core structures:
- where R1 R2, R3′, R3″, R4, R5, R6″, R6′, R7, R8, and R9 are, independently, selected from hydrogen or the same or different substituent groups having no more than 40 atoms,
- where at least one of R3′, R3″, R6″ and R6′ is a lipophilic substituent;
- where R9 in core structure (II) is a nonaromatic substituent.
Dependencies are inferred from the claim text.