US 9,029,107 B2 · Hydrolase protein

Abstract

It is an object of the present invention to provide a novel hydrolase, which is used when dialkyl 2-vinylcyclopropane-1,1-dicarboxylate is hydrolyzed with an enzyme, so as to efficiently obtain (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid that is useful as an intermediate for synthesizing therapeutic agents for hepatitis C. According to the present invention, there is provided a hydrolase protein, which consists of the amino acid sequence shown in any one of SEQ ID NOS. 2 to 5 and which has activity of catalyzing, at higher selectivity than the protein consisting of the amino acid sequence shown in SEQ ID NO. 1, a reaction of producing (1S,2S)-1-ethoxycarbonyl-2-vinylcyclopropanecarboxylic acid from diethyl 2-vinylcyclopropane-1,1-dicarboxylate.

Claims11

Dependent claims

A hydrolase protein capable of catalyzing the following reaction at higher selectivity than the protein consisting of the amino acid sequence shown in SEQ ID NO:

1:
which hydrolase protein comprises an amino acid sequence having 90% or more identity to SEQ ID NO:
2, and wherein one or more amino acids of the amino acid sequence are substituted at positions corresponding to one or more of amino acid residue numbers 70, 270, and 273 with other amino acids whose side chains are less bulky than those of wild-type amino acids.

Dependencies are inferred from the claim text.

Hydrolase protein

Life of this patent

Hydrolase protein

Life of this patent

Abstract

Claims11