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US 9,023,994 B2Granted
Immunoglobulin reduced in thrombogenic agents and preparation thereof
AssigneeOmrix Biopharmaceuticals Ltd.
InventorsRoni Mintz, Oleg Belyaev, Israel Nur, Liliana Bar, Roberto Meidler
Life of this patent
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Abstract
The invention relates to an immunoglobulin composition reduced in thrombogenic agents and to methods for its preparation. One method comprises subjecting an immunoglobulin containing solution to a negative cation exchanger chromatography at a pH in the range of higher than 3.8 to equal to or lower than 5.3. The solution can also be subjected to a negative anion exchanger chromatography at a pH in the range of 7 to 8.2.
Claims14
A method for preparing an immunoglobulin composition comprising the steps of:
- a) contacting an immunoglobulin solution obtained by Cohn Fractionation and/or Kistler-Nitschmann fractionation or a fraction thereof with an anion exchanger equilibrated at a pH in the range of 7 to 8.2, and a cation exchanger equilibrated at a pH in the range of higher than 3.8 to equal to or lower than 5.3 to allow prekallikrein activator (PKA) (“PKA”) to bind to the anion exchanger, and Factor XIa, Factor XI and/or Kallikrein to bind to the cation exchanger;
- and
- b) collecting the unbound fractions comprising immunoglobulin.
Dependencies are inferred from the claim text.