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US 9,023,797 B2Granted
Caprylate viral deactivation
AssigneeGrifols, S.A.
InventorsWytold Lebing, Doug Burns, Nathan Roth, Joann Hotta
Life of this patent
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Abstract
Described herein are methods for inactivating viruses using caprylate in solutions containing albumin.
Claims21
A method of preparing albumin from a solution comprising albumin, the method comprising:
- (i) adjusting the protein concentration of the solution to less than about 5%;
- (ii) adding caprylic acid (octanoic acid) or sodium caprylate to the solution obtained in step (i);
- (iii) adjusting pH of the solution obtained in step (ii) to about pH 5 or less;
- (iv) raising the temperature of the solution obtained in step (iii) to about 27-30° C.;
- and
- (v) incubating the solution obtained in step (iv) for about 30 min to 120 min.
3 more elements
A method of preparing albumin from a solution comprising albumin, the method comprising:
- (i) adjusting the protein concentration of the solution to less than about 5%;
- (ii) adding caprylic acid (octanoic acid) or sodium caprylate to a concentration of about 20 mM to the solution obtained in step (i);
- (iii) adjusting pH of the solution obtained in step (ii) to about pH 5 or less;
- (iv) raising the temperature of the solution obtained in step (iii) to about 27-30° C.;
- and
- (v) incubating the solution obtained in step (iv) for at least about 30 min to about 120 min.
3 more elements
A method of inactivating viruses in a solution comprising albumin, the method comprising:
- (i) adjusting the protein concentration of the solution to less than about 5%;
- (ii) adding caprylic acid (octanoic acid) or sodium caprylate to the solution obtained in step (i), wherein the caprylic acid or sodium caprylate concentration is greater than about 20 mM;
- (iii) raising the solution temperature of the solution obtained in step (ii) to greater than about 20° C.;
- iv) incubating the solution;
- v) filtering the solution;
- vi) performing ultrafiltration and diafiltration;
- vii) formulating and bulking the solution;
- and
- viii) sterilizing, filling, and pasteurizing the albumin.
6 more elements
A method of inactivating viruses in a solution comprising albumin, the method comprising:
- (i) adjusting the protein concentration of the solution to less than about 5%;
- (ii) adding caprylic acid (octanoic acid) or sodium caprylate to a concentration of about 20 mM to the solution obtained in step (i);
- (iii) raising the temperature of the solution obtained in step (ii) to about 27-30° C.;
- (iv) incubating the solution for at least 90 min;
- (v) filtering the solution;
- (vi) performing ultrafiltration and diafiltration;
- (vii) formulating and bulking the solution;
- and
- (viii) sterilizing, filling, and pasteurizing the albumin.
6 more elements
Dependencies are inferred from the claim text.