Enhanced citric acid production in Aspergillus with inactivated asparagine-linked glycosylation protein 3 (Alg3), and/or increased LaeA expression
AssigneeBattelle Memorial Institute
InventorsZiyu Dai, Scott E. Baker
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Abstract
Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.
Claims20
An isolated fungus comprising a genetic inactivation of an endogenous dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene, wherein the fungus is an Aspergillus species and the Alg3 gene is genetically inactivated by complete or partial deletion mutation or by insertional mutation, wherein the endogenous Alg3 gene prior to the genetic inactivation encodes a protein having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 2 or 4.
An isolated fungus comprising a genetic inactivation of an endogenous dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene, wherein the fungus is an Aspergillus species and the Alg3 gene is genetically inactivated by complete or partial deletion mutation or by insertional mutation, wherein the endogenous Alg3 gene prior to the genetic inactivation has at least 80% sequence identity to the polynucleotide of SEQ ID NO:
- 1 or 3.
An isolated fungus, comprising a genetic inactivation of an endogenous dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene, wherein the Alg3 gene is genetically inactivated by complete or partial deletion mutation or by insertional mutation, and wherein prior to genetic inactivation, the Alg3 gene encodes the protein of SEQ ID NO:
- 2 or SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.